Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김옥빈 | * |
dc.date.accessioned | 2019-04-16T16:30:11Z | - |
dc.date.available | 2019-04-16T16:30:11Z | - |
dc.date.issued | 2019 | * |
dc.identifier.issn | 1758-2229 | * |
dc.identifier.other | OAK-24596 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/249628 | - |
dc.description.abstract | Actinobacillus succinogenes is a natural succinate producer, which is the result of fumarate respiration. Succinate production from anaerobic growth with C 4 -dicarboxylates requires transporters catalysing uptake and efflux of C 4 -dicarboxylates. Transporter Asuc_1999 (DcuE) found in A. succinogenes belongs to the Dcu family and was considered the main transporter for fumarate respiration. However, deletion of dcuE affected l-malate uptake of A. succinogenes rather than fumarate uptake. DcuE complemented anaerobic growth of Escherichia coli on l-malate or fumarate; thus, the transporter was characterized in E. coli heterologously. Time-dependent uptake and competitive inhibition assays demonstrated that l-malate is the most preferred substrate for uptake by DcuE. The V max of DcuE for l-malate was 20.04 μmol/gDW·min with K m of 57 μM. The V max for l-malate was comparable to that for fumarate, whereas the K m for l-malate was 8 times lower than that for fumarate. The catalytic efficiency of DcuE for l-malate was 7.3-fold higher than that for fumarate, showing high efficiency and high affinity for l-malate. Furthermore, DcuE catalysed the reversible exchange of three C 4 -dicarboxylates – l-malate, fumarate and succinate – but the preferred substrate for uptake was l-malate. Under physiological conditions, the C 4 -dicarboxylates were reduced to succinate. Therefore, DcuE is proposed as the l-malate/succinate antiporter in A. succinogenes. © 2018 Society for Applied Microbiology and John Wiley & Sons Ltd | * |
dc.language | English | * |
dc.publisher | Wiley-Blackwell | * |
dc.title | High-affinity l-malate transporter DcuE of Actinobacillus succinogenes catalyses reversible exchange of C4-dicarboxylates | * |
dc.type | Article | * |
dc.relation.issue | 2 | * |
dc.relation.volume | 11 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 129 | * |
dc.relation.lastpage | 139 | * |
dc.relation.journaltitle | Environmental Microbiology Reports | * |
dc.identifier.doi | 10.1111/1758-2229.12719 | * |
dc.identifier.wosid | WOS:000461871000007 | * |
dc.identifier.scopusid | 2-s2.0-85059116898 | * |
dc.author.google | Rhie M.N. | * |
dc.author.google | Cho Y.B. | * |
dc.author.google | Lee Y.J. | * |
dc.author.google | Kim O.B. | * |
dc.contributor.scopusid | 김옥빈(15851079900) | * |
dc.date.modifydate | 20240301081003 | * |