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D-tyrosine negatively regulates melanin synthesis by competitively inhibiting tyrosinase activity
- Title
- D-tyrosine negatively regulates melanin synthesis by competitively inhibiting tyrosinase activity
- Authors
- Park J.; Jung H.; Kim K.; Lim K.-M.; Kim J.-Y.; Jho E.-H.; Oh E.-S.
- Ewha Authors
- 오억수; 임경민
- SCOPUS Author ID
- 오억수; 임경민
- Issue Date
- 2018
- Journal Title
- Pigment Cell and Melanoma Research
- ISSN
- 1755-1471
- Citation
- Pigment Cell and Melanoma Research vol. 31, no. 3, pp. 374 - 383
- Keywords
- D-tyrosine; L-tyrosine; melanin; melanogenesis; tyrosinase
- Publisher
- Blackwell Publishing Ltd
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Although L-tyrosine is well known for its melanogenic effect, the contribution of D-tyrosine to melanin synthesis was previously unexplored. Here, we reveal that, unlike L-tyrosine, D-tyrosine dose-dependently reduced the melanin contents of human MNT-1 melanoma cells and primary human melanocytes. In addition, 500 μM of D-tyrosine completely inhibited 10 μM L-tyrosine-induced melanogenesis, and both in vitro assays and L-DOPA staining MNT-1 cells showed that tyrosinase activity is reduced by D-tyrosine treatment. Thus, D-tyrosine appears to inhibit L-tyrosine-mediated melanogenesis by competitively inhibiting tyrosinase activity. Furthermore, we found that D-tyrosine inhibited melanogenesis induced by α-MSH treatment or UV irradiation, which are the most common environmental factors responsible for melanin synthesis. Finally, we confirmed that D-tyrosine reduced melanin synthesis in the epidermal basal layer of a 3D human skin model. Taken together, these data suggest that D-tyrosine negatively regulates melanin synthesis by inhibiting tyrosinase activity in melanocyte-derived cells. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
- DOI
- 10.1111/pcmr.12668
- Appears in Collections:
- 자연과학대학 > 생명과학전공 > Journal papers
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