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Production of stearidonic acid-rich triacylglycerol via a two-step enzymatic esterification

Title
Production of stearidonic acid-rich triacylglycerol via a two-step enzymatic esterification
Authors
Kim N.H.Kim H.Choi N.Kim Y.Kim B.H.Kim I.-H.
Ewha Authors
김양하
SCOPUS Author ID
김양하scopus
Issue Date
2019
Journal Title
Food Chemistry
ISSN
0308-8146JCR Link
Citation
vol. 270, pp. 332 - 337
Keywords
EsterificationLipaseLipozyme TL IMNovozym 435Stearidonic acidTriacylglycerol
Publisher
Elsevier Ltd
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
The aim of this study was to synthesize stearidonic acid (SDA)-rich triacylglycerol (TAG) via a two-step lipase-catalyzed esterification under vacuum. SDA-rich fatty acid, which was prepared from echium oil via Candida rugosa lipase-catalyzed selective esterification, was used as the substrate. Two different immobilized lipases, Novozym 435 from Candida antarctica and Lipozyme TL IM from Thermomyces lanuginosus, were employed for the synthesis of SDA-rich TAG. In the first step, Novozym 435-catalyzed esterification of the SDA-rich fatty acid with glycerol was carried out for 2 h. In the second step, Lipozyme TL IM-catalyzed esterification of the reaction mixture from the first step was performed for an additional 10 h. The optimal reaction conditions for the second step were a temperature of 65 °C, an enzyme loading of 20%, and a vacuum of 0.7 kPa. Consequently, the maximum TAG conversion of ca. 86.4 wt% was obtained after 12 h via a two-step lipase-catalyzed esterification. © 2018 Elsevier Ltd
DOI
10.1016/j.foodchem.2018.07.121
Appears in Collections:
신산업융합대학 > 식품영양학과 > Journal papers
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