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Selenite-induced apoptosis of osteoclasts mediated by the mitochondrial pathway
- Title
- Selenite-induced apoptosis of osteoclasts mediated by the mitochondrial pathway
- Authors
- Chung Y.W.; Kim T.S.; Lee S.Y.; Lee S.H.; Choi Y.; Kim N.; Min B.-M.; Jeong D.-W.; Kim I.Y.
- Ewha Authors
- 이수영
- SCOPUS Author ID
- 이수영
- Issue Date
- 2006
- Journal Title
- Toxicology Letters
- ISSN
- 0378-4274
- Citation
- Toxicology Letters vol. 160, no. 2, pp. 143 - 150
- Indexed
- SCI; SCIE; SCOPUS
- Document Type
- Article
- Abstract
- The possible effects of sodium selenite on mature osteoclasts were investigated. Incubation of osteoclast-like cells differentiated from RAW 264.7 cells with sodium selenite induced apoptosis as revealed by morphological changes, internucleosomal DNA fragmentation, and activation of caspase-3. Selenite also induced generation of the superoxide anion and reduced the number of free thiol groups in the osteoclast-like cells, suggestive of a shift to a more oxidizing intracellular environment. In addition, selenite induced protein aggregation by thiol cross-linking, loss of the mitochondrial membrane potential, and cytochrome c release in mitochondria isolated from the osteoclast-like cells. Finally, selenite-induced DNA fragmentation in osteoclasts was inhibited both by cyclosporin A, a blocker of the mitochondrial permeability transition pore, and by DEVD-CHO, a cell-permeable inhibitor of caspase-3. These results thus suggest that selenite induces apoptosis mediated by the mitochondrial pathway in mature osteoclasts. © 2005 Elsevier Ireland Ltd. All rights reserved.
- DOI
- 10.1016/j.toxlet.2005.06.019
- Appears in Collections:
- 자연과학대학 > 생명과학전공 > Journal papers
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