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Immobilization of bone morphogenetic protein-2 on a nanofibrous chitosan membrane for enhanced guided bone regeneration
- Title
- Immobilization of bone morphogenetic protein-2 on a nanofibrous chitosan membrane for enhanced guided bone regeneration
- Authors
- Park Y.J.; Kim K.H.; Lee J.Y.; Ku Y.; Lee S.J.; Min B.M.; Chung C.P.
- Ewha Authors
- 이승진
- SCOPUS Author ID
- 이승진
- Issue Date
- 2006
- Journal Title
- Biotechnology and Applied Biochemistry
- ISSN
- 0885-4513
- Citation
- Biotechnology and Applied Biochemistry vol. 43, no. 1, pp. 17 - 24
- Indexed
- SCI; SCIE; SCOPUS
- Document Type
- Article
- Abstract
- BMP-2 (bone morphogenetic protein-2) has been used in promoting bone formation and, in combination with various delivery carriers, in bone regeneration or bone tissue-engineering practice. In the present study, BMP-2 was immobilized directly on a GBR (guided bone-regenerative) membrane surface made of chitosan nanofibres, providing a bioactive surface that can enhance bone-regeneration capacity. The BMP-2-conjugated membrane surface retained bioactivity for up to 4 weeks of incubation, as well as holding over 50% of the initial BMP-2 attached. The BMP-2-conjugated surface increased osteoblastic cell attachment in accordance with the amount of BMP-2 conjugated. In addition, the BMP-2-conjugated chitosan membrane significantly promoted cell proliferation, alkaline phosphatase activity, as well as calcium deposition when compared with BMP-2-adsorbed membrane, indicating a stable localization of BMP-2 at the surface of the chitosan membrane. Taking these findings together, the conjugation of BMP-2 on a chitosan nanofibrous membrane would seen to be applicable for inducing significant and localized bone formation in future GBR procedures. © 2006 Portland Press Ltd.
- DOI
- 10.1042/BA20050075
- Appears in Collections:
- 약학대학 > 약학과 > Journal papers
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