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Selective patterning and immobilization of biomolecules within precisely-defined micro-reservoirs

Title
Selective patterning and immobilization of biomolecules within precisely-defined micro-reservoirs
Authors
Lee N.Y.Lim J.R.Kim Y.S.
Ewha Authors
김연상
Issue Date
2006
Journal Title
Biosensors and Bioelectronics
ISSN
0956-5663JCR Link
Citation
Biosensors and Bioelectronics vol. 21, no. 11, pp. 2188 - 2193
Indexed
SCI; SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Herein, we present the fabrication of well-defined micro-reservoirs and a simple strategy to immobilize biomolecules selectively inside the reservoirs. The micro-reservoirs are fabricated using a photocurable prepolymer, which enables the formation of concrete structures with high-fidelity, so that the reservoirs are spatially-segregated from each other by rigid physical barriers. For the directed binding of the protein, two steps are involved. First, poly(ethylene glycol) (PEG) is contact-printed on those areas where the protein binding is not desired, and next, protein binding is promoted where desired via carbodiimide chemistry. Fluorescein-tagged albumin is successfully immobilized inside the micro-reservoirs and microchannel arrays with high sensitivity, regardless of the sizes of the reservoirs and channels. The proposed system can be used for constructing multi-functional biosensors by immobilizing individual bioorganisms specifically in each micro-reservoir or microchannel. © 2005 Elsevier B.V. All rights reserved.
DOI
10.1016/j.bios.2005.11.003
Appears in Collections:
자연과학대학 > 화학·나노과학전공 > Journal papers
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