Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김연상 | - |
dc.date.accessioned | 2018-05-30T08:14:01Z | - |
dc.date.available | 2018-05-30T08:14:01Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.other | OAK-3287 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/243480 | - |
dc.description.abstract | Herein, we present the fabrication of well-defined micro-reservoirs and a simple strategy to immobilize biomolecules selectively inside the reservoirs. The micro-reservoirs are fabricated using a photocurable prepolymer, which enables the formation of concrete structures with high-fidelity, so that the reservoirs are spatially-segregated from each other by rigid physical barriers. For the directed binding of the protein, two steps are involved. First, poly(ethylene glycol) (PEG) is contact-printed on those areas where the protein binding is not desired, and next, protein binding is promoted where desired via carbodiimide chemistry. Fluorescein-tagged albumin is successfully immobilized inside the micro-reservoirs and microchannel arrays with high sensitivity, regardless of the sizes of the reservoirs and channels. The proposed system can be used for constructing multi-functional biosensors by immobilizing individual bioorganisms specifically in each micro-reservoir or microchannel. © 2005 Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.title | Selective patterning and immobilization of biomolecules within precisely-defined micro-reservoirs | - |
dc.type | Article | - |
dc.relation.issue | 11 | - |
dc.relation.volume | 21 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 2188 | - |
dc.relation.lastpage | 2193 | - |
dc.relation.journaltitle | Biosensors and Bioelectronics | - |
dc.identifier.doi | 10.1016/j.bios.2005.11.003 | - |
dc.identifier.wosid | WOS:000236959400023 | - |
dc.identifier.scopusid | 2-s2.0-33645848751 | - |
dc.author.google | Lee N.Y. | - |
dc.author.google | Lim J.R. | - |
dc.author.google | Kim Y.S. | - |
dc.contributor.scopusid | 김연상(8938854200) | - |
dc.date.modifydate | 20211210152421 | - |