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Synthesis of alpha-linolenic acid-rich triacylglycerol using a newly prepared immobilized lipase

Title
Synthesis of alpha-linolenic acid-rich triacylglycerol using a newly prepared immobilized lipase
Authors
Kim, HeejinChoi, NakyungOh, Se-WookKim, YanghaKim, Byung HeeKim, In-Hwan
Ewha Authors
김양하
SCOPUS Author ID
김양하scopus
Issue Date
2017
Journal Title
FOOD CHEMISTRY
ISSN
0308-8146JCR Link1873-7072JCR Link
Citation
vol. 237, pp. 654 - 658
Keywords
Duolite A568Immobilizationalpha-Linolenic acidLipaseThermomyces lanuginosusTriacylglycerol
Publisher
ELSEVIER SCI LTD
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
An alpha-linolenic acid (ALA)-rich triacylglycerol (TAG) was synthesized from an ALA-rich fatty acid (FA) from perilla oil and glycerol, using a newly prepared immobilized lipase under vacuum. The ALA-rich FA (purity >90 wt%) used as the substrate was prepared by urea complexation from perilla oil FAs. Liquid Lipozyme TL 100 L lipase from Thermomyces lanuginosus was used for immobilization. Nine different hydrophilic and hydrophobic carriers for immobilization were tested, and Duolite A568, which is a hydrophilic resin, was selected as the best carrier. This immobilized lipase was used to synthesize TAG by direct esterification under vacuum. The parameters investigated were temperature, enzyme loading, and vacuum level. The optimum reaction conditions were a temperature of 60 degrees C, an enzyme loading of 15% (based on the total weight of the substrate), and a vacuum of 0.7 kPa, respectively. The maximum conversion to TAG of ca. 88 wt% was obtained in 12 h under the optimum conditions. (C) 2017 Elsevier Ltd. All rights reserved.
DOI
10.1016/j.foodchem.2017.05.161
Appears in Collections:
신산업융합대학 > 식품영양학과 > Journal papers
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