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Cellular Antioxidant and Anti-Inflammatory Effects of Coffee Extracts with Different Roasting Levels

Title
Cellular Antioxidant and Anti-Inflammatory Effects of Coffee Extracts with Different Roasting Levels
Authors
Jung S.Kim M.H.Park J.H.Jeong Y.Ko K.S.
Ewha Authors
고광석
SCOPUS Author ID
고광석scopus
Issue Date
2017
Journal Title
Journal of Medicinal Food
ISSN
1096-620XJCR Link
Citation
vol. 20, no. 6, pp. 626 - 635
Keywords
Anti-inflammatoryAntioxidativeCoffeeHepatoprotectionRoasting degree
Publisher
Mary Ann Liebert Inc.
Indexed
SCI; SCIE; SCOPUS; KCI WOS scopus
Abstract
During roasting, major changes occur in the composition and physiological effects of coffee beans. In this study, in vitro antioxidant effects and anti-inflammatory effects of Coffea arabica green coffee extracts were investigated at different roasting levels corresponding to Light, Medium, City, and French roast. Total caffeine did not show huge difference according to roasting level, but total chlorogenic acid contents were higher in light roasted coffee extract than other roasted groups. In addition, light roasted coffee extract had the highest antioxidant activity in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. To determine the in vitro antioxidant property, coffee extracts were used to treat AML-12 cells. Intracellular glutathione (GSH) concentration and mRNA expression levels of genes related to GSH synthesis were negatively related to roasting levels. The anti-inflammatory effects of coffee extracts were investigated in lipopolysaccharide-treated RAW 264.7 macrophage cells. The cellular antioxidant activity of coffee extracts exhibited similar patterns as the AML-12 cells. The expression of mRNA for tumor necrosis factor-alpha and interleukin-6 was decreased in cells treated with the coffee extracts and the expression decreased with increasing roasting levels. These data suggest that coffee has physiological antioxidant and anti-inflammatory activities and these effects are negatively correlated with roasting levels in the cell models. © 2017, Mary Ann Liebert, Inc., and Korean Society of Food Science and Nutrition.
DOI
10.1089/jmf.2017.3935
Appears in Collections:
신산업융합대학 > 식품영양학과 > Journal papers
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