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Portable Fluorometer for Cyanobacteria Detection

Title
Portable Fluorometer for Cyanobacteria Detection
Authors
Kim, Seung-HoonHe, YingLee, Eun-HeeKim, Jeong-HoPark, Sung Min
Ewha Authors
김정호박성민이은희
SCOPUS Author ID
김정호scopus; 박성민scopus; 이은희scopusscopus
Issue Date
2017
Journal Title
IEEE SENSORS JOURNAL
ISSN
1530-437XJCR Link1558-1748JCR Link
Citation
vol. 17, no. 8, pp. 2377 - 2384
Keywords
CyanobacteriadetectionfluorescenceMicrocystis aeruginosaquantum dotstransducer kit
Publisher
IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
Indexed
SCIE; SCOPUS WOS
Abstract
A portable system for fluorescence measurement has been developed for cyanobacteria detection. Exploiting commercially available low-cost photodiodes and a single 9 V supply, the system was able to measure two fluorescence emissions simultaneously. Output voltages resulting from the fluorescence intensity were measured by utilizing two quantum dots of QD(565) and QD(655). These quantum dots were employed into a NanoGene assay for mcyD gene (synthetase of toxic microcystin) detection of Microcystis aeruginosa. We investigated various operational parameters (e. g., distance, angle between laser emission and sample, and so on) for the proposed system. Measurements indicated the highest output voltage signal was obtained at a distance of 0.5 cm with the right angle (90 degrees) between the laser source and a sample vial. Also, the mcyD gene hybridized with QD(565) and QD(655) was quantitatively detected by the proposed detection system, i. e., the normalized output voltage (QD(655)/QD(565)) was linearly proportional to the mcyD gene copy numbers (y = 0.41 log x + 6.6, r(2) = 0.92). Finally, the quantitative data were transferred wirelessly to the readout via Bluetooth.
DOI
10.1109/JSEN.2017.2672993
Appears in Collections:
엘텍공과대학 > 전자공학과 > Journal papers
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