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A Reversible Fluorescent Probe for Real-Time Quantitative Monitoring of Cellular Glutathione

Title
A Reversible Fluorescent Probe for Real-Time Quantitative Monitoring of Cellular Glutathione
Authors
Liu Z.Zhou X.Miao Y.Hu Y.Kwon N.Wu X.Yoon J.
Ewha Authors
윤주영
SCOPUS Author ID
윤주영scopus
Issue Date
2017
Journal Title
Angewandte Chemie - International Edition
ISSN
1433-7851JCR Link
Citation
vol. 56, no. 21, pp. 5812 - 5816
Keywords
antioxidantsfluorescent probesgluthionineratiometric probesredox homeostasis
Publisher
Wiley-VCH Verlag
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
The ability to monitor and quantify glutathione (GSH) in live cells is essential in order to gain a detailed understanding of GSH-related pathological events. However, owing to their irreversible response mechanisms, most existing fluorescent GSH probes are not suitable for this purpose. We have developed a ratiometric fluorescent probe (QG-1) for quantitatively monitoring cellular GSH. The probe responds specifically and reversibility to GSH with an ideal dissociation constant (Kd) of 2.59 mm and a fast response time (t1/2=5.82 s). We also demonstrate that QG-1 detection of GSH is feasible in a model protein system. QG-1 was found to have extremely low cytotoxicity and was applied to determine the GSH concentration in live HeLa cells (5.40±0.87 mm). © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
DOI
10.1002/anie.201702114
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자연과학대학 > 화학·나노과학전공 > Journal papers
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