Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 윤주영 | * |
dc.date.accessioned | 2017-06-13T01:06:13Z | - |
dc.date.available | 2017-06-13T01:06:13Z | - |
dc.date.issued | 2017 | * |
dc.identifier.issn | 1433-7851 | * |
dc.identifier.other | OAK-20680 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/235256 | - |
dc.description.abstract | The ability to monitor and quantify glutathione (GSH) in live cells is essential in order to gain a detailed understanding of GSH-related pathological events. However, owing to their irreversible response mechanisms, most existing fluorescent GSH probes are not suitable for this purpose. We have developed a ratiometric fluorescent probe (QG-1) for quantitatively monitoring cellular GSH. The probe responds specifically and reversibility to GSH with an ideal dissociation constant (Kd) of 2.59 mm and a fast response time (t1/2=5.82 s). We also demonstrate that QG-1 detection of GSH is feasible in a model protein system. QG-1 was found to have extremely low cytotoxicity and was applied to determine the GSH concentration in live HeLa cells (5.40±0.87 mm). © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim | * |
dc.language | English | * |
dc.publisher | Wiley-VCH Verlag | * |
dc.subject | antioxidants | * |
dc.subject | fluorescent probes | * |
dc.subject | gluthionine | * |
dc.subject | ratiometric probes | * |
dc.subject | redox homeostasis | * |
dc.title | A Reversible Fluorescent Probe for Real-Time Quantitative Monitoring of Cellular Glutathione | * |
dc.type | Article | * |
dc.relation.issue | 21 | * |
dc.relation.volume | 56 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 5812 | * |
dc.relation.lastpage | 5816 | * |
dc.relation.journaltitle | Angewandte Chemie - International Edition | * |
dc.identifier.doi | 10.1002/anie.201702114 | * |
dc.identifier.wosid | WOS:000400755800027 | * |
dc.identifier.scopusid | 2-s2.0-85016615531 | * |
dc.author.google | Liu Z. | * |
dc.author.google | Zhou X. | * |
dc.author.google | Miao Y. | * |
dc.author.google | Hu Y. | * |
dc.author.google | Kwon N. | * |
dc.author.google | Wu X. | * |
dc.author.google | Yoon J. | * |
dc.contributor.scopusid | 윤주영(7403587371) | * |
dc.date.modifydate | 20240118162450 | * |