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dc.contributor.author윤주영*
dc.date.accessioned2017-06-13T01:06:13Z-
dc.date.available2017-06-13T01:06:13Z-
dc.date.issued2017*
dc.identifier.issn1433-7851*
dc.identifier.otherOAK-20680*
dc.identifier.urihttps://dspace.ewha.ac.kr/handle/2015.oak/235256-
dc.description.abstractThe ability to monitor and quantify glutathione (GSH) in live cells is essential in order to gain a detailed understanding of GSH-related pathological events. However, owing to their irreversible response mechanisms, most existing fluorescent GSH probes are not suitable for this purpose. We have developed a ratiometric fluorescent probe (QG-1) for quantitatively monitoring cellular GSH. The probe responds specifically and reversibility to GSH with an ideal dissociation constant (Kd) of 2.59 mm and a fast response time (t1/2=5.82 s). We also demonstrate that QG-1 detection of GSH is feasible in a model protein system. QG-1 was found to have extremely low cytotoxicity and was applied to determine the GSH concentration in live HeLa cells (5.40±0.87 mm). © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim*
dc.languageEnglish*
dc.publisherWiley-VCH Verlag*
dc.subjectantioxidants*
dc.subjectfluorescent probes*
dc.subjectgluthionine*
dc.subjectratiometric probes*
dc.subjectredox homeostasis*
dc.titleA Reversible Fluorescent Probe for Real-Time Quantitative Monitoring of Cellular Glutathione*
dc.typeArticle*
dc.relation.issue21*
dc.relation.volume56*
dc.relation.indexSCIE*
dc.relation.indexSCOPUS*
dc.relation.startpage5812*
dc.relation.lastpage5816*
dc.relation.journaltitleAngewandte Chemie - International Edition*
dc.identifier.doi10.1002/anie.201702114*
dc.identifier.wosidWOS:000400755800027*
dc.identifier.scopusid2-s2.0-85016615531*
dc.author.googleLiu Z.*
dc.author.googleZhou X.*
dc.author.googleMiao Y.*
dc.author.googleHu Y.*
dc.author.googleKwon N.*
dc.author.googleWu X.*
dc.author.googleYoon J.*
dc.contributor.scopusid윤주영(7403587371)*
dc.date.modifydate20240118162450*
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자연과학대학 > 화학·나노과학전공 > Journal papers
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