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Istamycin aminoglycosides profiling and their characterization in Streptomyces tenjimariensis ATCC 31603 culture using high-performance liquid chromatography with tandem mass spectrometry

Title
Istamycin aminoglycosides profiling and their characterization in Streptomyces tenjimariensis ATCC 31603 culture using high-performance liquid chromatography with tandem mass spectrometry
Authors
Nguyen Huu HoangNguyen Lan HuongKim, ByulSohng, Jae KyungYoon, Yeo JoonPark, Je Won
Ewha Authors
윤여준
SCOPUS Author ID
윤여준scopus
Issue Date
2016
Journal Title
JOURNAL OF SEPARATION SCIENCE
ISSN
1615-9306JCR Link1615-9314JCR Link
Citation
vol. 39, no. 24, pp. 4712 - 4722
Keywords
Chiral columnsEpimeric mixturesIstamycin aminoglycosideStreptomyces tenjimariensis
Publisher
WILEY-V C H VERLAG GMBH
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
A high-performance liquid chromatography with electrospray ionization ion trap tandem mass spectrometry method was developed and validated for the robust profiling and characterization of biosynthetic congeners in the 2-deoxy-aminocyclitol istamycin pathway, from the fermentation broth of Streptomyces tenjimariensis ATCC 31603. Gradient elution on an Acquity CSH C-18 column was performed with a gradient of 5 mM aqueous pentafluoropropionic acid and 50% acetonitrile. Sixteen natural istamycin congeners were profiled and quantified in descending order; istamycin A, istamycin B, istamycin A(0), istamycin B-0, istamycin B-1, istamycin A(1), istamycin C, istamycin A(2), istamycin C-1, istamycin C-0, istamycin X-0, istamycin A(3), istamycin Y-0, istamycin B-3, and istamycin FU-10 plus istamycin AP. In addition, a total of five sets of 1- or 3-epimeric pairs were chromatographically separated using a macrocyclic glycopeptide-bonded chiral column. The lower limit of quantification of istamycin-A present in S. tenjimariensis fermentation was estimated to be 2.2 ng/mL. The simultaneous identification of a wide range of 2-deoxy-aminocyclitol-type istamycin profiles from bacterial fermentation was determined for the first time by employing high-performance liquid chromatography with tandem mass spectrometry analysis and the separation of istamycin epimers.
DOI
10.1002/jssc.201600925
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자연과학대학 > 화학·나노과학전공 > Journal papers
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