Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 윤여준 | - |
dc.date.accessioned | 2017-02-15T08:02:09Z | - |
dc.date.available | 2017-02-15T08:02:09Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 1615-9306 | - |
dc.identifier.issn | 1615-9314 | - |
dc.identifier.other | OAK-20110 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/234539 | - |
dc.description.abstract | A high-performance liquid chromatography with electrospray ionization ion trap tandem mass spectrometry method was developed and validated for the robust profiling and characterization of biosynthetic congeners in the 2-deoxy-aminocyclitol istamycin pathway, from the fermentation broth of Streptomyces tenjimariensis ATCC 31603. Gradient elution on an Acquity CSH C-18 column was performed with a gradient of 5 mM aqueous pentafluoropropionic acid and 50% acetonitrile. Sixteen natural istamycin congeners were profiled and quantified in descending order; istamycin A, istamycin B, istamycin A(0), istamycin B-0, istamycin B-1, istamycin A(1), istamycin C, istamycin A(2), istamycin C-1, istamycin C-0, istamycin X-0, istamycin A(3), istamycin Y-0, istamycin B-3, and istamycin FU-10 plus istamycin AP. In addition, a total of five sets of 1- or 3-epimeric pairs were chromatographically separated using a macrocyclic glycopeptide-bonded chiral column. The lower limit of quantification of istamycin-A present in S. tenjimariensis fermentation was estimated to be 2.2 ng/mL. The simultaneous identification of a wide range of 2-deoxy-aminocyclitol-type istamycin profiles from bacterial fermentation was determined for the first time by employing high-performance liquid chromatography with tandem mass spectrometry analysis and the separation of istamycin epimers. | - |
dc.language | English | - |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.subject | Chiral columns | - |
dc.subject | Epimeric mixtures | - |
dc.subject | Istamycin aminoglycoside | - |
dc.subject | Streptomyces tenjimariensis | - |
dc.title | Istamycin aminoglycosides profiling and their characterization in Streptomyces tenjimariensis ATCC 31603 culture using high-performance liquid chromatography with tandem mass spectrometry | - |
dc.type | Article | - |
dc.relation.issue | 24 | - |
dc.relation.volume | 39 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 4712 | - |
dc.relation.lastpage | 4722 | - |
dc.relation.journaltitle | JOURNAL OF SEPARATION SCIENCE | - |
dc.identifier.doi | 10.1002/jssc.201600925 | - |
dc.identifier.wosid | WOS:000393030400005 | - |
dc.identifier.scopusid | 2-s2.0-85005996224 | - |
dc.author.google | Nguyen Huu Hoang | - |
dc.author.google | Nguyen Lan Huong | - |
dc.author.google | Kim, Byul | - |
dc.author.google | Sohng, Jae Kyung | - |
dc.author.google | Yoon, Yeo Joon | - |
dc.author.google | Park, Je Won | - |
dc.contributor.scopusid | 윤여준(7402126465) | - |
dc.date.modifydate | 20210915144031 | - |