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In vivo near-infrared imaging and phototherapy of tumors using a cathepsin B-activated fluorescent probe
- In vivo near-infrared imaging and phototherapy of tumors using a cathepsin B-activated fluorescent probe
- Chen, Xiaoqiang; Lee, Dayoung; Yu, Sungsook; Kim, Gyoungmi; Lee, Songyi; Cho, Yejin; Jeong, Haengdueng; Nam, Ki Taek; Yoon, Juyoung
- Ewha Authors
- 윤주영; Chen Xiaoqiang
- SCOPUS Author ID
- Issue Date
- Journal Title
- 0142-9612; 1878-5905
- vol. 122, pp. 130 - 140
- Specific phototherapy; NIR bioimaging; Enzyme-activated fluorescence; Multiple functional probe
- ELSEVIER SCI LTD
- SCI; SCIE; SCOPUS
- The development of multifunctional reagents for simultaneous specific near-infrared (NIR) imaging and phototherapy of tumors is of great significance. This work describes the design of a cathepsin B-activated fluorescent probe (CyA-P-CyB) and its applications as an NIR imaging probe for tumor cells and as a phototherapy reagent for tumors. In vitro experiments demonstrated that CyA-P-CyB was activated via the cleavage of a peptide linker by cathepsin B in tumor cells to produce fluorescence in the NIR region based on a FRET mechanism. MTT assays showed that the phototoxicity of CyA-P-CyB toward cells depended on the activity of cathepsin B, and the probe exhibited specific phototoxicity toward tumor cells. CyA-P-CyB was also successfully applied to the in vivo imaging and phototherapy of tumors. Histological analysis indicated that CyA-P-CyB had no cytotoxic effects on seven mouse tissues (lung, liver, heart, kidney, pancreas, spleen and brain) after the CyA-P-CyB treatment and laser irradiation. (C) 2017 Elsevier Ltd. All rights reserved.
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