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In vivo near-infrared imaging and phototherapy of tumors using a cathepsin B-activated fluorescent probe

Title
In vivo near-infrared imaging and phototherapy of tumors using a cathepsin B-activated fluorescent probe
Authors
Chen, XiaoqiangLee, DayoungYu, SungsookKim, GyoungmiLee, SongyiCho, YejinJeong, HaengduengNam, Ki TaekYoon, Juyoung
Ewha Authors
윤주영Chen Xiaoqiang
SCOPUS Author ID
윤주영scopus
Issue Date
2017
Journal Title
BIOMATERIALS
ISSN
0142-9612JCR Link1878-5905JCR Link
Citation
vol. 122, pp. 130 - 140
Keywords
Specific phototherapyNIR bioimagingEnzyme-activated fluorescenceMultiple functional probe
Publisher
ELSEVIER SCI LTD
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
The development of multifunctional reagents for simultaneous specific near-infrared (NIR) imaging and phototherapy of tumors is of great significance. This work describes the design of a cathepsin B-activated fluorescent probe (CyA-P-CyB) and its applications as an NIR imaging probe for tumor cells and as a phototherapy reagent for tumors. In vitro experiments demonstrated that CyA-P-CyB was activated via the cleavage of a peptide linker by cathepsin B in tumor cells to produce fluorescence in the NIR region based on a FRET mechanism. MTT assays showed that the phototoxicity of CyA-P-CyB toward cells depended on the activity of cathepsin B, and the probe exhibited specific phototoxicity toward tumor cells. CyA-P-CyB was also successfully applied to the in vivo imaging and phototherapy of tumors. Histological analysis indicated that CyA-P-CyB had no cytotoxic effects on seven mouse tissues (lung, liver, heart, kidney, pancreas, spleen and brain) after the CyA-P-CyB treatment and laser irradiation. (C) 2017 Elsevier Ltd. All rights reserved.
DOI
10.1016/j.biomaterials.2017.01.020
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자연과학대학 > 화학·나노과학전공 > Journal papers
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