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Hydrogen peroxide induces the death astrocytes through the down-regulation of the constitutive nuclear factor-kappaB activity

Title
Hydrogen peroxide induces the death astrocytes through the down-regulation of the constitutive nuclear factor-kappaB activity
Authors
Choi J.-J.Choi J.Kang C.-D.Chen X.Wu C.-F.Ko K.H.Kim W.-K.
Ewha Authors
김원기
Issue Date
2007
Journal Title
Free Radical Research
ISSN
1071-5762JCR Link
Citation
vol. 41, no. 5, pp. 555 - 562
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Nuclear factor-kappaB (NF-κB) has a dual role in the promotion or attenuation of cell death. Here, we demonstrated the role of NF-κB in the H2O2-induced death of astrocytes. H2O2 evoked the release of lactate dehydrogenase (LDH), a marker of cell death, and concomitantly decreased the DNA binding and transcriptional activity of NF-κ3ba;B in cultured astrocytes. H2O2-induced astrocyte death was markedly increased by the co-treatment with pyrrolidinedithiocarbamate, an NF-κB inhibitor. Moreover, the elevation of constitutive NF-κB activity by overexpressing p65 NF-κB subunit attenuated H2O2 toxicity, whereas NF-κB inhibition by overexpressing Iκba;B potentiated the toxicity. NF-κB activity and H2O2 cytotoxicity was further found to be dependent on cell density. Compared with astrocytes in low cell density, those in high cell density exhibited a higher constitutive NF-κB activity and a stronger resistance to H2O2 cytotoxicity. These results indicate that the constitutive activity of NF-κB in astrocytes is required for their survival under oxidative stress such as H2O2.
DOI
10.1080/10715760601173010
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자연과학대학 > 화학·나노과학전공 > Journal papers
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