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Green tea catechin enhances cholesterol 7α-hydroxylase gene expression in HepG2 cells

Title
Green tea catechin enhances cholesterol 7α-hydroxylase gene expression in HepG2 cells
Authors
Lee M.-S.Park J.-Y.Freake H.Kwun I.-S.Kim Y.
Ewha Authors
김양하
SCOPUS Author ID
김양하scopus
Issue Date
2008
Journal Title
British Journal of Nutrition
ISSN
0007-1145JCR Link
Citation
vol. 99, no. 6, pp. 1182 - 1185
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Green tea catechins are known to have hypocholesterolaemic effects in animals and human subjects. In the present study, we investigated the effects of green tea catechins on the mRNA level and promoter activity of hepatic cholesterol 7α-hydroxylase (CYP7A1), the rate-limiting enzyme in the conversion of cholesterol to bile acids, in human hepatoma cells. Real-time PCR assays showed that different catechins, (-)-epicatechin gallate (ECG), (-)-epigallocatechin-3-gallate (EGCG), (-)-epigallocatechin (EGC) and (-)-epicatechin (EC), up regulated the CYP7A1 mRNA level by 5.5-, 4.2-, 2.9- and 1.9-fold, respectively, compared with the control. The -1312/+358 bp of the CYP7A1 promoter was subcloned into the pGL3 basic vector that includes luciferase as a reporter gene. ECG or EGCG significantly increased CYP7A1 promoter activity by 6.0- or 4.0-fold, respectively, compared with the control. Also, EGCG stimulated CYP7A1 at both mRNA level and promoter activity in a dose-dependent manner. These results suggest that the expression of the CYP7A1 gene may be directly regulated by green tea catechins at the transcriptional level. © The Authors 2007.
DOI
10.1017/S0007114507864816
Appears in Collections:
신산업융합대학 > 식품영양학과 > Journal papers
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