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Self-assembly of protein nanoarrays on block copolymer templates

Title
Self-assembly of protein nanoarrays on block copolymer templates
Authors
Lau K.H.A.Bang J.Kim D.H.Knoll W.
Ewha Authors
김동하
SCOPUS Author ID
김동하scopus
Issue Date
2008
Journal Title
Advanced Functional Materials
ISSN
1616-301XJCR Link
Citation
vol. 18, no. 20, pp. 3148 - 3157
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
There is considerable interest in developing functional protein arrays on the nanoscale for high-throughput protein-based array technology, and for the study of biomolecular and cell interactions at the physical scale of the biomolecules. To these ends, self-assembly based techniques may be desirable for the nanopatterning of proteins on large sample areas without the use of lithography equipment. We present a fast, general approach for patterning proteins (and potentially other biomolecules) on the nanoscale, which takes advantage of the ability of block copolymers to self-assemble into ordered surface nanopatterns with defined chemical heterogeneity. We demonstrate nanoarrays of immunoglobulin and bovine serum albumin on polystyreneblock -poly(methyl methacrylate) templates, and illustrate the applicability of our technique through immunoassays and DNA sensing performed on the protein nanoarrays. Furthermore, we show that the pattern formation mechanism is a nanoscale effect originating from a combination of fluid flow forces and geometric restrictions templated by an underlying nanopattern with a difference in protein adsorption behavior on adjacent, chemically distinct surfaces. This understanding may provide a framework for extending the patterning approach to other proteins and material systems. © 2008 WILEY-VCH Verlag GmbH & Co. KGaA.
DOI
10.1002/adfm.200800487
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자연과학대학 > 화학·나노과학전공 > Journal papers
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