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Bacteroides fragilis Enterotoxin Upregulates Heme Oxygenase-1 in Intestinal Epithelial Cells via a Mitogen-Activated Protein Kinase- and NF-kappa B-Dependent Pathway, Leading to Modulation of Apoptosis

Title
Bacteroides fragilis Enterotoxin Upregulates Heme Oxygenase-1 in Intestinal Epithelial Cells via a Mitogen-Activated Protein Kinase- and NF-kappa B-Dependent Pathway, Leading to Modulation of Apoptosis
Authors
Ko, Su HyukRho, Da JeongJeon, Jong IkKim, Young-JeonWoo, Hyun AeLee, Yun KyungKim, Jung Mogg
Ewha Authors
우현애
SCOPUS Author ID
우현애scopus
Issue Date
2016
Journal Title
INFECTION AND IMMUNITY
ISSN
0019-9567JCR Link1098-5522JCR Link
Citation
vol. 84, no. 9, pp. 2541 - 2554
Publisher
AMER SOC MICROBIOLOGY
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
The Bacteroides fragilis enterotoxin (BFT), a virulence factor of enterotoxigenic B. fragilis (ETBF), interacts with intestinal epithelial cells and can provoke signals that induce mucosal inflammation. Although expression of heme oxygenase-1 (HO-1) is associated with regulation of inflammatory responses, little is known about HO-1 induction in ETBF infection. This study was conducted to investigate the effect of BFT on HO-1 expression in intestinal epithelial cells. Stimulation of intestinal epithelial cells with BFT resulted in upregulated expression of HO-1. BFT activated transcription factors such as NF-kappa B, AP-1, and Nrf2 in intestinal epithelial cells. Upregulation of HO-1 in intestinal epithelial cells was dependent on activated I kappa B kinase (IKK)-NF-kappa B signals. However, suppression of Nrf2 or AP-1 signals in intestinal epithelial cells did not result in significant attenuation of BFT-induced HO-1 expression. HO-1 induction via IKK-NF-kappa B in intestinal epithelial cells was regulated by p38 mitogen-activated protein kinases (MAPKs). Furthermore, suppression of HO-1 activity led to increased apoptosis in BFT-stimulated epithelial cells. These results suggest that a signaling pathway involving p38 MAPK-IKK-NF-kappa B in intestinal epithelial cells is required for HO-1 induction during exposure to BFT. Following this induction, increased HO-1 expression may regulate the apoptotic process in responses to BFT stimulation.
DOI
10.1128/IAI.00191-16
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약학대학 > 약학과 > Journal papers
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