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Gene expression regulation by agonist-independent constitutive signaling of Melanocortin-1 receptor

Title
Gene expression regulation by agonist-independent constitutive signaling of Melanocortin-1 receptor
Authors
Seong I.Kim J.
Ewha Authors
김재상
SCOPUS Author ID
김재상scopus
Issue Date
2014
Journal Title
Endocrinology and Metabolism
ISSN
2093-596XJCR Link
Citation
vol. 29, no. 2, pp. 179 - 184
Keywords
ChemokinesMelanocortinMelanomaMigrationReceptorType 1
Publisher
Korean Endocrine Society
Indexed
SCOPUS; KCI scopus
Abstract
Background: Melanocortin-1 receptor (Mc1r), a key signaling receptor for melanogenesis, has been reported to mediate migration of B16F10 melanoma cells. Interestingly, this activity appears to be a part of the constitutive signaling of Mc1r. Methods: We carried out small interfering RNA-mediated knock-down of Mc1r on murine melanoma B16F10 cells and performed microarray analysis to characterize changes in the gene expression profile. Results: We isolated 22 and four genes whose expression decreased and increased, respectively, by 2.5-fold or higher as the result of Mc1r knock-down. Several down-regulated genes have been proposed to be involved in cell migration. Among these genes are several members of the chemokine gene family. Conclusion: We provide a gene set for further functional analyses of Mc1r. The Mc1r target genes we present may be particularly relevant for understanding the ligand-independent activity of Mc1r. Further examination of the mode of action may lead to novel strategies in regulating the migration and metastasis of melanoma cells. © 2014 Korean Endocrine Society.
DOI
10.3803/EnM.2014.29.2.179
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자연과학대학 > 생명과학전공 > Journal papers
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