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약학대학
약학과
Journal papers
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Bioorthogonal Copper Free Click Chemistry for Labeling and Tracking of Chondrocytes In Vivo
Title
Bioorthogonal Copper Free Click Chemistry for Labeling and Tracking of Chondrocytes In Vivo
Authors
Yoon H.I.
;
Yhee J.Y.
;
Na J.H.
;
Lee S.
;
Lee H.
;
Kang S.-W.
;
Chang H.
;
Ryu J.H.
;
Kwon I.C.
;
Cho Y.W.
;
Kim K.
Ewha Authors
이혁진
SCOPUS Author ID
이혁진
Issue Date
2016
Journal Title
Bioconjugate Chemistry
ISSN
1043-1802
Citation
Bioconjugate Chemistry vol. 27, no. 4, pp. 927 - 936
Publisher
American Chemical Society
Indexed
SCI; SCIE; SCOPUS
Document Type
Article
Abstract
Establishment of an appropriate cell labeling and tracking method is essential for the development of cell-based therapeutic strategies. Here, we are introducing a new method for cell labeling and tracking by combining metabolic gylcoengineering and bioorthogonal copper-free Click chemistry. First, chondrocytes were treated with tetraacetylated N-azidoacetyl-D-mannosamine (Ac4ManNAz) to generate unnatural azide groups (-N3) on the surface of the cells. Subsequently, the unnatural azide groups on the cell surface were specifically conjugated with near-infrared fluorescent (NIRF) dye-tagged dibenzyl cyclooctyne (DBCO-650) through bioorthogonal copper-free Click chemistry. Importantly, DBCO-650-labeled chondrocytes presented strong NIRF signals with relatively low cytotoxicity and the amounts of azide groups and DBCO-650 could be easily controlled by feeding different amounts of Ac4ManNAz and DBCO-650 to the cell culture system. For the in vivo cell tracking, DBCO-650-labeled chondrocytes (1 × 106 cells) seeded on the 3D scaffold were subcutaneously implanted into mice and the transplanted DBCO-650-labeled chondrocytes could be effectively tracked in the prolonged time period of 4 weeks using NIRF imaging technology. Furthermore, this new cell labeling and tracking technology had minimal effect on cartilage formation in vivo. (Figure Presented). © 2016 American Chemical Society.
DOI
10.1021/acs.bioconjchem.6b00010
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