Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 이혁진 | * |
dc.date.accessioned | 2016-08-29T12:08:47Z | - |
dc.date.available | 2016-08-29T12:08:47Z | - |
dc.date.issued | 2016 | * |
dc.identifier.issn | 1043-1802 | * |
dc.identifier.other | OAK-18572 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/231538 | - |
dc.description.abstract | Establishment of an appropriate cell labeling and tracking method is essential for the development of cell-based therapeutic strategies. Here, we are introducing a new method for cell labeling and tracking by combining metabolic gylcoengineering and bioorthogonal copper-free Click chemistry. First, chondrocytes were treated with tetraacetylated N-azidoacetyl-D-mannosamine (Ac4ManNAz) to generate unnatural azide groups (-N3) on the surface of the cells. Subsequently, the unnatural azide groups on the cell surface were specifically conjugated with near-infrared fluorescent (NIRF) dye-tagged dibenzyl cyclooctyne (DBCO-650) through bioorthogonal copper-free Click chemistry. Importantly, DBCO-650-labeled chondrocytes presented strong NIRF signals with relatively low cytotoxicity and the amounts of azide groups and DBCO-650 could be easily controlled by feeding different amounts of Ac4ManNAz and DBCO-650 to the cell culture system. For the in vivo cell tracking, DBCO-650-labeled chondrocytes (1 × 106 cells) seeded on the 3D scaffold were subcutaneously implanted into mice and the transplanted DBCO-650-labeled chondrocytes could be effectively tracked in the prolonged time period of 4 weeks using NIRF imaging technology. Furthermore, this new cell labeling and tracking technology had minimal effect on cartilage formation in vivo. (Figure Presented). © 2016 American Chemical Society. | * |
dc.language | English | * |
dc.publisher | American Chemical Society | * |
dc.title | Bioorthogonal Copper Free Click Chemistry for Labeling and Tracking of Chondrocytes In Vivo | * |
dc.type | Article | * |
dc.relation.issue | 4 | * |
dc.relation.volume | 27 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 927 | * |
dc.relation.lastpage | 936 | * |
dc.relation.journaltitle | Bioconjugate Chemistry | * |
dc.identifier.doi | 10.1021/acs.bioconjchem.6b00010 | * |
dc.identifier.wosid | WOS:000374812600011 | * |
dc.identifier.scopusid | 2-s2.0-84965082415 | * |
dc.author.google | Yoon H.I. | * |
dc.author.google | Yhee J.Y. | * |
dc.author.google | Na J.H. | * |
dc.author.google | Lee S. | * |
dc.author.google | Lee H. | * |
dc.author.google | Kang S.-W. | * |
dc.author.google | Chang H. | * |
dc.author.google | Ryu J.H. | * |
dc.author.google | Kwon I.C. | * |
dc.author.google | Cho Y.W. | * |
dc.author.google | Kim K. | * |
dc.contributor.scopusid | 이혁진(55233457200) | * |
dc.date.modifydate | 20240220111730 | * |