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Whole cell bioconversion of ricinoleic acid to 12-ketooleic acid by recombinant Corynebacterium glutamicum-based biocatalyst

Title
Whole cell bioconversion of ricinoleic acid to 12-ketooleic acid by recombinant Corynebacterium glutamicum-based biocatalyst
Authors
Lee B.Lee S.Kim H.Jeong K.Park J.Park K.Lee J.
Ewha Authors
박진병
SCOPUS Author ID
박진병scopus
Issue Date
2015
Journal Title
Journal of Microbiology and Biotechnology
ISSN
1017-7825JCR Link
Citation
vol. 25, no. 4, pp. 452 - 458
Keywords
12-Ketooleic acidCorynebacterium glutamicumRicinoleic acidSecondary alcohol dehydrogenaseWhole cell bioconversion
Publisher
Korean Society for Microbiolog and Biotechnology
Indexed
SCIE; SCOPUS; KCI WOS scopus
Abstract
The biocatalytic efficiency of recombinant Corynebacterium glutamicum ATCC 13032 expressing the secondary alcohol dehydrogenase of Micrococcus luteus NCTC2665 was studied. Recombinant C. glutamicum converts ricinoleic acid to a product, identified by gas chromatography/mass spectrometry as 12-ketooleic acid (12-oxo-cis-9-octadecenoic acid). The effects of pH, reaction temperature, and non-ionic detergent on recombinant C. glutamiucm whole cell bioconversion were examined. The determined optimal conditions for production of 12-ketooleic acid are pH 8.0, 35°C, and 0.05 g/l Tween80. Under these conditions, recombinant C. glutamicum produces 3.3 mM 12-ketooleic acid, with a 72% (mol/mol) maximum conversion yield, and 1.1 g/l/h volumetric productivity in 2 h; and 3.9 mM 12- ketooleic acid, with a 74% (mol/mol) maximum conversion yield, and 0.69 g/l/h maximum volumetric productivity in 4 h of fermentation. This study constitutes the first report of significant production of 12-ketooleic acid using a recombinant Corynebacterium glutamicumbased biocatalyst. © 2015 by The Korean Society for Microbiology and Biotechnology.
DOI
10.4014/jmb.1501.01001
Appears in Collections:
엘텍공과대학 > 식품공학전공 > Journal papers
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