Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박진병 | * |
dc.date.accessioned | 2016-08-29T12:08:06Z | - |
dc.date.available | 2016-08-29T12:08:06Z | - |
dc.date.issued | 2015 | * |
dc.identifier.issn | 1017-7825 | * |
dc.identifier.other | OAK-14930 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/230568 | - |
dc.description.abstract | The biocatalytic efficiency of recombinant Corynebacterium glutamicum ATCC 13032 expressing the secondary alcohol dehydrogenase of Micrococcus luteus NCTC2665 was studied. Recombinant C. glutamicum converts ricinoleic acid to a product, identified by gas chromatography/mass spectrometry as 12-ketooleic acid (12-oxo-cis-9-octadecenoic acid). The effects of pH, reaction temperature, and non-ionic detergent on recombinant C. glutamiucm whole cell bioconversion were examined. The determined optimal conditions for production of 12-ketooleic acid are pH 8.0, 35°C, and 0.05 g/l Tween80. Under these conditions, recombinant C. glutamicum produces 3.3 mM 12-ketooleic acid, with a 72% (mol/mol) maximum conversion yield, and 1.1 g/l/h volumetric productivity in 2 h; and 3.9 mM 12- ketooleic acid, with a 74% (mol/mol) maximum conversion yield, and 0.69 g/l/h maximum volumetric productivity in 4 h of fermentation. This study constitutes the first report of significant production of 12-ketooleic acid using a recombinant Corynebacterium glutamicumbased biocatalyst. © 2015 by The Korean Society for Microbiology and Biotechnology. | * |
dc.language | English | * |
dc.publisher | Korean Society for Microbiolog and Biotechnology | * |
dc.subject | 12-Ketooleic acid | * |
dc.subject | Corynebacterium glutamicum | * |
dc.subject | Ricinoleic acid | * |
dc.subject | Secondary alcohol dehydrogenase | * |
dc.subject | Whole cell bioconversion | * |
dc.title | Whole cell bioconversion of ricinoleic acid to 12-ketooleic acid by recombinant Corynebacterium glutamicum-based biocatalyst | * |
dc.type | Article | * |
dc.relation.issue | 4 | * |
dc.relation.volume | 25 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.index | KCI | * |
dc.relation.startpage | 452 | * |
dc.relation.lastpage | 458 | * |
dc.relation.journaltitle | Journal of Microbiology and Biotechnology | * |
dc.identifier.doi | 10.4014/jmb.1501.01001 | * |
dc.identifier.wosid | WOS:000353664000005 | * |
dc.identifier.scopusid | 2-s2.0-84948688399 | * |
dc.author.google | Lee B. | * |
dc.author.google | Lee S. | * |
dc.author.google | Kim H. | * |
dc.author.google | Jeong K. | * |
dc.author.google | Park J. | * |
dc.author.google | Park K. | * |
dc.author.google | Lee J. | * |
dc.contributor.scopusid | 박진병(15036390700) | * |
dc.date.modifydate | 20240322114808 | * |