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Lysate of probiotic lactobacillus plantarum K8 modulate the mucosal inflammatory system in dextran sulfate sodium-induced colitic rats

Title
Lysate of probiotic lactobacillus plantarum K8 modulate the mucosal inflammatory system in dextran sulfate sodium-induced colitic rats
Authors
Ahn Y.-S.Park M.Y.Shin J.-H.Kim J.Y.Kwon O.
Ewha Authors
권오란
SCOPUS Author ID
권오란scopus
Issue Date
2014
Journal Title
Korean Journal for Food Science of Animal Resources
ISSN
1225-8563JCR Link
Citation
vol. 34, no. 6, pp. 829 - 835
Keywords
ColitisLysate of Lactobacillus plantarum K8Pro-inflammatory cytokinesToll-like receptor 2
Publisher
Korean Society for Food Science of Animal Resources
Indexed
SCIE; SCOPUS; KCI WOS scopus
Abstract
Inflammatory bowel disease (IBD) is caused by dysregulation of colon mucosal immunity and mucosal epithelial barrier function. Recent studies have reported that lipoteichoic acid (LTA) from Lactobacillus plantarum K8 reduces excessive production of pro-inflammatory cytokine. In this study, we investigated the preventive effects of lysate of Lb. plantarum K8 in dextran sulfate sodium (DSS)-induced colitis. Male Sprague-Dawley rats were orally pretreated with lysate of Lb. plantarum K8 (low dose or high dose) or live Lb. plantarum K8 prior to the induction of colitis using 4% DSS. Disease progression was monitored by assessment of disease activity index (DAI). Histological changes of colonic tissues were evaluated by hematoxylin and eosin (HE) staining. Tumor necrosis factor-alpha (TNF-a), interleukin-6 (IL-6) levels were measured using enzyme-linked immunosorbent assay (ELISA). The colon mRNA expressions of TNF-a, IL-6, and toll like receptor-2 (TLR-2) were examined by quantitative real-time-transcription polymerase chain reaction (qPCR). Lysate of Lb. plantarum K8 suppressed colon shortening, edema, mucosal damage, and the loss of DSS-induced crypts. The groups that received lysate of Lb. plantarum K8 exhibited significantly decreased levels of the pro-inflammatory cytokines TNF-a and IL-6 in the colon. Interestingly, colonic expression of toll like receptor-2 mRNA in the high-dose lysate of Lb. plantarum K8 group increased significantly. Our study demonstrates the protective effects of oral lysate of Lb. plantarum K8 administration on DSS-induced colitis via the modulation of pro-inflammatory mediators of the mucosal immune system.
DOI
10.5851/kosfa.2014.34.6.829
Appears in Collections:
신산업융합대학 > 식품영양학과 > Journal papers
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