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Re-engineering of genetic circuit for 2-deoxystreptamine (2-DOS) biosynthesis in Escherichia coli BL21 (DE3)

Title
Re-engineering of genetic circuit for 2-deoxystreptamine (2-DOS) biosynthesis in Escherichia coli BL21 (DE3)
Authors
Chaudhary A.K.Park J.W.Yoon Y.J.Kim B.-G.Sohng J.K.
Ewha Authors
윤여준
SCOPUS Author ID
윤여준scopus
Issue Date
2013
Journal Title
Biotechnology Letters
ISSN
0141-5492JCR Link
Citation
vol. 35, no. 2, pp. 285 - 293
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Various approaches for monocistronic constructions of genetic circuits have been designed for metabolite production but there has been no attempt to apply such methodology for aminoglycosides biosynthesis. Here, a simple and commercially available bio-part, despite the current trend focusing on the standardized BioBricks bio-parts available in the registry, is used. A 181-bp nucleotide fragment was designed for the efficient construction of an expression vector for monocistronic assembly of genes. Furthermore, a single vector with multi-monocistronic assembled genes for 2-deoxystreptamine (2-DOS) synthesis was constructed for production in engineered Escherichia coli. The working efficiency of model vector was concluded by reporter assay whereas the expressions of biosynthesis genes were confirmed by RT-PCR and SDS-PAGE. Production of 2-DOS was confirmed by TLC, LC-ELSD, and ESI-MS/MS. © 2012 Springer Science+Business Media Dordrecht.
DOI
10.1007/s10529-012-1077-2
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자연과학대학 > 화학·나노과학전공 > Journal papers
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