Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 윤여준 | - |
dc.date.accessioned | 2016-08-28T10:08:20Z | - |
dc.date.available | 2016-08-28T10:08:20Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.other | OAK-9674 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/223361 | - |
dc.description.abstract | Various approaches for monocistronic constructions of genetic circuits have been designed for metabolite production but there has been no attempt to apply such methodology for aminoglycosides biosynthesis. Here, a simple and commercially available bio-part, despite the current trend focusing on the standardized BioBricks bio-parts available in the registry, is used. A 181-bp nucleotide fragment was designed for the efficient construction of an expression vector for monocistronic assembly of genes. Furthermore, a single vector with multi-monocistronic assembled genes for 2-deoxystreptamine (2-DOS) synthesis was constructed for production in engineered Escherichia coli. The working efficiency of model vector was concluded by reporter assay whereas the expressions of biosynthesis genes were confirmed by RT-PCR and SDS-PAGE. Production of 2-DOS was confirmed by TLC, LC-ELSD, and ESI-MS/MS. © 2012 Springer Science+Business Media Dordrecht. | - |
dc.language | English | - |
dc.title | Re-engineering of genetic circuit for 2-deoxystreptamine (2-DOS) biosynthesis in Escherichia coli BL21 (DE3) | - |
dc.type | Article | - |
dc.relation.issue | 2 | - |
dc.relation.volume | 35 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 285 | - |
dc.relation.lastpage | 293 | - |
dc.relation.journaltitle | Biotechnology Letters | - |
dc.identifier.doi | 10.1007/s10529-012-1077-2 | - |
dc.identifier.wosid | WOS:000313972300020 | - |
dc.identifier.scopusid | 2-s2.0-84872768662 | - |
dc.author.google | Chaudhary A.K. | - |
dc.author.google | Park J.W. | - |
dc.author.google | Yoon Y.J. | - |
dc.author.google | Kim B.-G. | - |
dc.author.google | Sohng J.K. | - |
dc.contributor.scopusid | 윤여준(7402126465) | - |
dc.date.modifydate | 20210708161345 | - |