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Synthesis of monoacylglycerol containing pinolenic acid via stepwise esterification using a cold active lipase

Title
Synthesis of monoacylglycerol containing pinolenic acid via stepwise esterification using a cold active lipase
Authors
Pyo Y.-G.Hong S.I.Kim Y.Kim B.H.Kim I.-H.
Ewha Authors
김양하
SCOPUS Author ID
김양하scopus
Issue Date
2012
Journal Title
Biotechnology Progress
ISSN
8756-7938JCR Link
Citation
vol. 28, no. 5, pp. 1218 - 1224
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
High purity monoacylglycerol (MAG) containing pinolenic acid was synthesized via stepwise esterification of glycerol and fatty acids from pine nut oil using a cold active lipase from Penicillium camembertii as a biocatalyst. Effects of temperature, molar ratio, water content, enzyme loading, and vacuum on the synthesis of MAG by lipase-catalyzed esterification of glycerol and fatty acid from pine nut oil were investigated. Diacylglycerol (DAG) as well as MAG increased significantly when temperature was increased from 20 to 40°C. At a molar ratio of 1:1, MAG content decreased because of the significant increase in DAG content. Water has a profound influence on both MAG and DAG content through the entire course of reaction. The reaction rate increased significantly as enzyme loading increased up to 600 units. Vacuum was an effective method to reduce DAG content. The optimum temperature, molar ratio, water content, enzyme loading, vacuum, and reaction time were 20°C, 1:5 (fatty acid to glycerol), 2%, 600 units, 5 torr, and 24 h, respectively. MAG content further increased via lipase-catalyzed second step esterification at subzero temperature. P. camembertii lipase exhibited esterification activity up to -30°C. © 2012 American Institute of Chemical Engineers (AIChE).
DOI
10.1002/btpr.1596
Appears in Collections:
신산업융합대학 > 식품영양학과 > Journal papers
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