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Synthesis of CLA-enriched TAG by Candida antarctica lipase under vacuum

Title
Synthesis of CLA-enriched TAG by Candida antarctica lipase under vacuum
Authors
Hong S.I.Kim Y.Yoon S.W.Cho S.Y.Kim I.-H.
Ewha Authors
김양하
SCOPUS Author ID
김양하scopus
Issue Date
2012
Journal Title
European Journal of Lipid Science and Technology
ISSN
1438-7697JCR Link
Citation
vol. 114, no. 9, pp. 1044 - 1051
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Conjugated linoleic acid (CLA)-enriched triacylglycerol (TAG) of 90wt% was successfully synthesized in 10h by direct esterification of glycerol and CLA using an immobilized lipase from Candida antarctica under vacuum. The best operating conditions for the synthesis of TAG were investigated according to the three parameters of temperature, enzyme loading, and vacuum. The synthesis of TAG increased with increasing temperature but it did not significantly change above 60°C (p>0.05). The increase of enzyme loading lead to an enhanced conversion of TAG, but enzyme loading of more than 10% (based on the total weight of the substrates) was not effective. Moreover, when vacuum increased, the conversion of TAG increased, but the conversion rate decreased when the vacuum level was too high. The best combination of temperature, enzyme loading, and vacuum level were 60°C, 10% of the total weight of the substrates, and 0.4kPa, respectively. During the initial 6h of reaction, Candida antarctica lipase had more selectivity for 10t,12c-CLA than 9c,11t-CLA onto the glycerol backbone, and a preference for the incorporation at the sn-1,3 positions of glycerol rather than at the sn-2 position. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
DOI
10.1002/ejlt.201100233
Appears in Collections:
신산업융합대학 > 식품영양학과 > Journal papers
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