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Over-Expression of a RhoA-Specific Guanine Nucleotide Exchange Factor, p190RhoGEF, in Mouse Dendritic Cells Negatively Regulates Cellular Responses to Bacterial Lipopolysaccharide

Title
Over-Expression of a RhoA-Specific Guanine Nucleotide Exchange Factor, p190RhoGEF, in Mouse Dendritic Cells Negatively Regulates Cellular Responses to Bacterial Lipopolysaccharide
Authors
Seul H.J.Ahn Y.R.Song H.M.Ha Y.J.Lee J.R.
Ewha Authors
이종란
SCOPUS Author ID
이종란scopus
Issue Date
2012
Journal Title
Molecules and Cells
ISSN
1016-8478JCR Link
Citation
vol. 34, no. 2, pp. 159 - 164
Indexed
SCI; SCIE; SCOPUS; KCI WOS scopus
Abstract
We studied the role of a RhoA-specific guanine nucleotide exchange factor (p190RhoGEF) in dendritic cells (DCs), using transgenic (TG) mice that over-express a full gene of p190RhoGEF under the control of an invariant chain promoter. TG mice lacked localization of activated DCs to the T cell zone in the spleen and had reduced serum levels of IL-6 in response to lipopolysaccharide (LPS) injection. DCs from these mice also showed reduced surface expression of CD86, CD40, and CD205, but not MHCII, as well as a reduced capability to uptake antigen. Moreover, chemokinedriven migration and secretion of IL-6, but not of IL-12, were impaired after LPS-stimulation of TG DCs. Collectively, these results suggest that over-expressing p190RhoGEF negatively regulates conventional DC function in response to bacterial LPS infection. © The Korean Society for Molecular and Cellular Biology. All rights reserved.
DOI
10.1007/s10059-012-0055-9
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자연과학대학 > 생명과학전공 > Journal papers
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