View : 712 Download: 0
A MAP kinase pathway is implicated in the pseudohyphal induction by Hydrogen peroxide in candica albicans
- Title
- A MAP kinase pathway is implicated in the pseudohyphal induction by Hydrogen peroxide in candica albicans
- Authors
- Srinivasa K.; Kim J.; Yee S.; Kim W.; Choi W.
- Ewha Authors
- 최원자
- SCOPUS Author ID
- 최원자
![scopus](/images/layout/icon2.png)
- Issue Date
- 2012
- Journal Title
- Molecules and Cells
- ISSN
- 1016-8478
- Citation
- Molecules and Cells vol. 33, no. 2, pp. 183 - 193
- Indexed
- SCI; SCIE; SCOPUS; KCI
![scopus](/images/layout/scopus2.gif)
- Document Type
- Article
- Abstract
- Hydrogen peroxide (H2O2) functions as a ubiquitous intracellular messenger besides as an oxidative stress molecule. This dual role is based on the distinct cellular responses against different concentrations of H2O2. Previously, we demonstrated that both low (> 1 mM) and high (4-10 mM) doses of exogenous H2O2 induce filamentous growth with distinct cell morphology and growth rate in Candida albicans, suggesting the different transcription response. In this study, we revealed that the sub-toxic and toxic levels of H2O2 indeed induced pseudohyphae, but not true hyphae. Supporting this, several hyphae-specific genes that are expressed in true hyphae induced by serum were not detected in either sub-toxic or toxic H2O2 condition. A DNA microarray analysis was conducted to reveal the transcription profiles in cells treated with sub-toxic and toxic conditions of H2O2. Under the sub-toxic condition, a small number of genes involved in cell proliferation and metabolism were up-regulated, whereas a large number of genes were up-regulated in the toxic condition where the genes required for growth and proliferation were selectively restricted. For pseudohyphal induction by sub-toxic H2O2, Cek1 MAPK activating the transcription factor Cph1 was shown to be important. The absence of expression of several hyphae-specific genes known to be downstream targets of Cph1-signaling pathway for true hyphae formation suggests that the Cek1-mediated signaling pathway is not solely responsible for pseudohyphal formation by subtoxic H 2O2 and, but instead, complex networking pathway may exists by the activation of different regulators. © 2012 KSMCB.
- DOI
- 10.1007/s10059-012-2244-y
- Appears in Collections:
- 자연과학대학 > 생명과학전공 > Journal papers
- Files in This Item:
There are no files associated with this item.
- Export
- RIS (EndNote)
- XLS (Excel)
- XML