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Glycosylation of various flavonoids by recombinant oleandomycin glycosyltransferase from Streptomyces antibioticus in batch and repeated batch modes

Title
Glycosylation of various flavonoids by recombinant oleandomycin glycosyltransferase from Streptomyces antibioticus in batch and repeated batch modes
Authors
Choi S.H.Ryu M.Yoon Y.J.Kim D.-M.Lee E.Y.
Ewha Authors
윤여준
SCOPUS Author ID
윤여준scopus
Issue Date
2012
Journal Title
Biotechnology Letters
ISSN
0141-5492JCR Link
Citation
vol. 34, no. 3, pp. 499 - 505
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
An oleandomycin glycosyltransferase (OleD GT) gene from Streptomyces antibioticus was functionally expressed in Escherichia coli BL21 (DE3) with various molecular chaperones. The purified recombinant OleD GT catalyzed glycosylation of various flavonoids: apigenin, chrysin, daidzein, genistein, kaempferol, luteolin, 4-methylumbelliferone, naringenin, quercetin and resveratrol with UDP-glucose. 4.6 μg OleD GT was readily immobilized onto 1 mg hybrid nanoparticles of Fe 3O 4/silica/NiO on the basis of the affinity between His-tag and NiO nanoparticles with retention of 90% activity. In batch reaction, more than 90% naringenin (20 μM) was converted to its glycoside in 5 h. The immobilized OleD GT was efficiently reused for seven times whilst maintaining >60% of the residual activity in repeated glycosylation of naringenin. © 2011 Springer Science+Business Media B.V.
DOI
10.1007/s10529-011-0789-z
Appears in Collections:
자연과학대학 > 화학·나노과학전공 > Journal papers
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