Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 윤여준 | - |
dc.date.accessioned | 2016-08-28T12:08:03Z | - |
dc.date.available | 2016-08-28T12:08:03Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.other | OAK-8555 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/222441 | - |
dc.description.abstract | An oleandomycin glycosyltransferase (OleD GT) gene from Streptomyces antibioticus was functionally expressed in Escherichia coli BL21 (DE3) with various molecular chaperones. The purified recombinant OleD GT catalyzed glycosylation of various flavonoids: apigenin, chrysin, daidzein, genistein, kaempferol, luteolin, 4-methylumbelliferone, naringenin, quercetin and resveratrol with UDP-glucose. 4.6 μg OleD GT was readily immobilized onto 1 mg hybrid nanoparticles of Fe 3O 4/silica/NiO on the basis of the affinity between His-tag and NiO nanoparticles with retention of 90% activity. In batch reaction, more than 90% naringenin (20 μM) was converted to its glycoside in 5 h. The immobilized OleD GT was efficiently reused for seven times whilst maintaining >60% of the residual activity in repeated glycosylation of naringenin. © 2011 Springer Science+Business Media B.V. | - |
dc.language | English | - |
dc.title | Glycosylation of various flavonoids by recombinant oleandomycin glycosyltransferase from Streptomyces antibioticus in batch and repeated batch modes | - |
dc.type | Article | - |
dc.relation.issue | 3 | - |
dc.relation.volume | 34 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 499 | - |
dc.relation.lastpage | 505 | - |
dc.relation.journaltitle | Biotechnology Letters | - |
dc.identifier.doi | 10.1007/s10529-011-0789-z | - |
dc.identifier.wosid | WOS:000301296300013 | - |
dc.identifier.scopusid | 2-s2.0-84856516948 | - |
dc.author.google | Choi S.H. | - |
dc.author.google | Ryu M. | - |
dc.author.google | Yoon Y.J. | - |
dc.author.google | Kim D.-M. | - |
dc.author.google | Lee E.Y. | - |
dc.contributor.scopusid | 윤여준(7402126465) | - |
dc.date.modifydate | 20210708161345 | - |