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The matrix metalloproteinase-7 regulates the extracellular shedding of syndecan-2 from colon cancer cells

Title
The matrix metalloproteinase-7 regulates the extracellular shedding of syndecan-2 from colon cancer cells
Authors
Choi S.Kim J.-Y.Park J.H.Lee S.-T.Han I.-O.Oh E.-S.
Ewha Authors
오억수
SCOPUS Author ID
오억수scopus
Issue Date
2012
Journal Title
Biochemical and Biophysical Research Communications
ISSN
0006-291XJCR Link
Citation
vol. 417, no. 4, pp. 1260 - 1264
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
The cell surface heparan sulfate proteoglycan syndecan-2 regulates the activation of matrix metalloproteinase-7 (MMP-7) as a docking receptor. Here, we demonstrate the role of MMP-7 on syndecan-2 shedding in colon cancer cells. Western blot analysis showed that shed syndecan-2 was found in the culture media from various colon cancer cells. Overexpression of MMP-7 enhanced syndecan-2 shedding, whereas the opposite was true when MMP-7 levels were knocked-down using small inhibitory RNAs. Consistently, HT29 cells treated with MMP-7, but neither MMP-2 nor MMP-9, showed increased shed syndecan-2 in a time- and concentration-dependent manner. Furthermore, MALDI-TOF MS analysis and N-terminal amino acid sequencing revealed that MMP-7 cleaved both recombinant syndecan-2 and an endogenously glycosylated syndecan-2 ectodomain in the N-terminus at Leu 149 residue in vitro. Taken together, the data suggest that MMP-7 directly mediates shedding of syndecan-2 from colon cancer cells. © 2011 Elsevier Inc.
DOI
10.1016/j.bbrc.2011.12.120
Appears in Collections:
자연과학대학 > 생명과학전공 > Journal papers
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