Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 오억수 | - |
dc.date.accessioned | 2016-08-28T12:08:54Z | - |
dc.date.available | 2016-08-28T12:08:54Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.other | OAK-8452 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/222348 | - |
dc.description.abstract | The cell surface heparan sulfate proteoglycan syndecan-2 regulates the activation of matrix metalloproteinase-7 (MMP-7) as a docking receptor. Here, we demonstrate the role of MMP-7 on syndecan-2 shedding in colon cancer cells. Western blot analysis showed that shed syndecan-2 was found in the culture media from various colon cancer cells. Overexpression of MMP-7 enhanced syndecan-2 shedding, whereas the opposite was true when MMP-7 levels were knocked-down using small inhibitory RNAs. Consistently, HT29 cells treated with MMP-7, but neither MMP-2 nor MMP-9, showed increased shed syndecan-2 in a time- and concentration-dependent manner. Furthermore, MALDI-TOF MS analysis and N-terminal amino acid sequencing revealed that MMP-7 cleaved both recombinant syndecan-2 and an endogenously glycosylated syndecan-2 ectodomain in the N-terminus at Leu 149 residue in vitro. Taken together, the data suggest that MMP-7 directly mediates shedding of syndecan-2 from colon cancer cells. © 2011 Elsevier Inc. | - |
dc.language | English | - |
dc.title | The matrix metalloproteinase-7 regulates the extracellular shedding of syndecan-2 from colon cancer cells | - |
dc.type | Article | - |
dc.relation.issue | 4 | - |
dc.relation.volume | 417 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 1260 | - |
dc.relation.lastpage | 1264 | - |
dc.relation.journaltitle | Biochemical and Biophysical Research Communications | - |
dc.identifier.doi | 10.1016/j.bbrc.2011.12.120 | - |
dc.identifier.wosid | WOS:000300196100026 | - |
dc.identifier.scopusid | 2-s2.0-84862784281 | - |
dc.author.google | Choi S. | - |
dc.author.google | Kim J.-Y. | - |
dc.author.google | Park J.H. | - |
dc.author.google | Lee S.-T. | - |
dc.author.google | Han I.-O. | - |
dc.author.google | Oh E.-S. | - |
dc.contributor.scopusid | 오억수(7101967153) | - |
dc.date.modifydate | 20190901081003 | - |