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dc.contributor.author오억수-
dc.date.accessioned2016-08-28T12:08:54Z-
dc.date.available2016-08-28T12:08:54Z-
dc.date.issued2012-
dc.identifier.issn0006-291X-
dc.identifier.otherOAK-8452-
dc.identifier.urihttps://dspace.ewha.ac.kr/handle/2015.oak/222348-
dc.description.abstractThe cell surface heparan sulfate proteoglycan syndecan-2 regulates the activation of matrix metalloproteinase-7 (MMP-7) as a docking receptor. Here, we demonstrate the role of MMP-7 on syndecan-2 shedding in colon cancer cells. Western blot analysis showed that shed syndecan-2 was found in the culture media from various colon cancer cells. Overexpression of MMP-7 enhanced syndecan-2 shedding, whereas the opposite was true when MMP-7 levels were knocked-down using small inhibitory RNAs. Consistently, HT29 cells treated with MMP-7, but neither MMP-2 nor MMP-9, showed increased shed syndecan-2 in a time- and concentration-dependent manner. Furthermore, MALDI-TOF MS analysis and N-terminal amino acid sequencing revealed that MMP-7 cleaved both recombinant syndecan-2 and an endogenously glycosylated syndecan-2 ectodomain in the N-terminus at Leu 149 residue in vitro. Taken together, the data suggest that MMP-7 directly mediates shedding of syndecan-2 from colon cancer cells. © 2011 Elsevier Inc.-
dc.languageEnglish-
dc.titleThe matrix metalloproteinase-7 regulates the extracellular shedding of syndecan-2 from colon cancer cells-
dc.typeArticle-
dc.relation.issue4-
dc.relation.volume417-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.startpage1260-
dc.relation.lastpage1264-
dc.relation.journaltitleBiochemical and Biophysical Research Communications-
dc.identifier.doi10.1016/j.bbrc.2011.12.120-
dc.identifier.wosidWOS:000300196100026-
dc.identifier.scopusid2-s2.0-84862784281-
dc.author.googleChoi S.-
dc.author.googleKim J.-Y.-
dc.author.googlePark J.H.-
dc.author.googleLee S.-T.-
dc.author.googleHan I.-O.-
dc.author.googleOh E.-S.-
dc.contributor.scopusid오억수(7101967153)-
dc.date.modifydate20190901081003-
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자연과학대학 > 생명과학전공 > Journal papers
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