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Chondrocyte 3D-culture in RGD-modified crosslinked hydrogel with temperature-controllable modulus

Title
Chondrocyte 3D-culture in RGD-modified crosslinked hydrogel with temperature-controllable modulus
Authors
Lee H.Choi B.G.Moon H.J.Choi J.Park K.Jeong B.Han D.K.
Ewha Authors
정병문
SCOPUS Author ID
정병문scopus
Issue Date
2012
Journal Title
Macromolecular Research
ISSN
1598-5032JCR Link
Citation
vol. 20, no. 1, pp. 106 - 111
Indexed
SCI; SCIE; SCOPUS; KCI WOS scopus
Abstract
Based on the thermosensitivity of Pluronic® F127 and the cytointeractability of Arg-Gly-Asp (RGD) sequences, RGD-modified F127 dimethacrylate (FM-RGD) hydrogel was investigated as a 3-dimensional culture matrix for chondrocytes. Chondrocytes were encapsulated in the hydrogel by radical copolymerization of FM-RGD and FM in the presence of cells. The FM-RGD hydrogel modulus could be modulated by temperature variation from 15 °C (1,300 Pa) to the culture temperature of 37 °C (8,900 Pa), at which the hydrogel provided a condrocyte-compatible microenvironment. Compared with the hydrogel prepared from F127 dimethacrylate (FM) without RGD, the RGD-modified hydrogel produced significant (p<0.01) improvements in cell proliferation, DNA production, and viability while allowing the chondrocytes to maintain their original spherical phenotypes. © 2012 The Polymer Society of Korea and Springer Netherlands.
DOI
10.1007/s13233-012-0074-6
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자연과학대학 > 화학·나노과학전공 > Journal papers
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