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Gastrokine 1 functions as a tumor suppressor by inhibition of epithelial-mesenchymal transition in gastric cancers
- Gastrokine 1 functions as a tumor suppressor by inhibition of epithelial-mesenchymal transition in gastric cancers
- Yoon J.H.; Kang Y.H.; Choi Y.J.; Park I.S.; Nam S.W.; Lee J.Y.; Lee Y.S.; Park W.S.
- Ewha Authors
- SCOPUS Author ID
- Issue Date
- Journal Title
- Journal of Cancer Research and Clinical Oncology
- Journal of Cancer Research and Clinical Oncology vol. 137, no. 11, pp. 1697 - 1704
- SCI; SCIE; SCOPUS
- Document Type
- Purpose: Gastrokine 1 (GKN1) plays an important role in the gastric mucosal defense mechanism and also acts as a functional gastric tumor suppressor. The specific aim of this study was to determine the molecular mechanisms underlying GKN1 tumor suppressor activity in the progression of gastric cancers. Methods: We examined the effect of GKN1 on epithelial-mesenchymal transition (EMT) and cell migration in GKN1-transfected and recombinant GKN1-treated AGS gastric cancer cells using in vitro wound healing, microchemotaxis, and invasion assays. Results: In GKN1-transfected AGS cells, we observed inhibition of cell migration and invasion in wound healing, transwell and Matrigel assay. Also, GKN1-transfected and recombinant GKN1-treated AGS cells showed decreased levels of ROS and expression of phosphatidylinositol 3-kinase (PI3K)/Akt pathway proteins, concomitant with re-expression of E-cadherin and decreased expression of cytoplasmic and nuclear expression of β-catenin, slug, snail, fibronectin, and vimentin. Conclusions: These data suggest that the GKN1 gene may play an important role in the progression of sporadic gastric cancers via inhibition of EMT and cancer cell migration. © 2011 Springer-Verlag.
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