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Neuroprotective effect of Cu,Zn-superoxide dismutase fused to a TCTP-derived protein transduction domain

Title
Neuroprotective effect of Cu,Zn-superoxide dismutase fused to a TCTP-derived protein transduction domain
Authors
Lee J.Kim S.Shin D.H.Kim H.-J.Lee K.
Ewha Authors
김화정이경림신동해
SCOPUS Author ID
김화정scopus; 이경림scopus; 신동해scopus
Issue Date
2011
Journal Title
European Journal of Pharmacology
ISSN
0014-2999JCR Link
Citation
vol. 666, no. 41277, pp. 87 - 92
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Previously, we have reported that a 10-amino acid peptide (MIIYRDLISH) derived from the NH2-terminus of the human translationally controlled tumor protein (TCTP) functions as a protein transduction domain (PTD). In this study, we evaluated the transduction ability of SOD fused to TCTP-PTD (TCTP-SOD) into various cell lines. We also evaluated its ability to protect cells against paraquat-induced cell damage, in vitro and its neuroprotective effect in vivo against kainic acid-induced neuronal damage in an animal model. TCTP-SOD was transduced into various cell lines in a dose- and time-dependent manner without cytotoxic effect. Furthermore, TCTP-SOD showed cytoprotective activity in SH-SY5Y cells, and intraperitoneally, injected TCTP-SOD was delivered into the mouse brain and protected the cells in the hippocampal region against the damage induced by kainic acid. We propose TCTP-SOD as a potential candidate drug for treatment of brain diseases. © 2011 Elsevier B.V.
DOI
10.1016/j.ejphar.2011.05.040
Appears in Collections:
약학대학 > 약학과 > Journal papers
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