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Enzymatic synthesis of lysophosphatidylcholine containing CLA from sn-glycero-3-phosphatidylcholine (GPC) under vacuum

Title
Enzymatic synthesis of lysophosphatidylcholine containing CLA from sn-glycero-3-phosphatidylcholine (GPC) under vacuum
Authors
Hong S.I.Kim Y.Kim C.-T.Kim I.-H.
Ewha Authors
김양하
SCOPUS Author ID
김양하scopus
Issue Date
2011
Journal Title
Food Chemistry
ISSN
0308-8146JCR Link
Citation
vol. 129, no. 1, pp. 1 - 6
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Lysophosphatidylcholine (LPC) was successfully synthesised by enzyme-catalysed esterification of sn-glycero-3-phosphatidylcholine (GPC) with the acid form of conjugated linoleic acid (CLA), using a vacuum system. GPC was prepared from phosphatidylcholine (PC) by alkaline deacylation using a methanolic tetra-butylammonium hydroxide solution. Five enzymes were tested as biocatalysts for direct esterification, and lipases were found to be more effective than phospholipases for production of LPC. Amongst the enzymes tested, Novozym 435, from Candida antarctica, gave the highest yield of LPC. The optimal temperature, molar ratio of substrate, and vacuum were 40°C, 1:50 (GPC to CLA), and 1 mm Hg, respectively. The maximum yield (70 mol%) of LPC was obtained under optimal conditions after a 48 h reaction time. © 2011 Elsevier Ltd. All rights reserved.
DOI
10.1016/j.foodchem.2011.04.038
Appears in Collections:
신산업융합대학 > 식품영양학과 > Journal papers
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