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The cell penetrating ability of the proapoptotic peptide, KLAKLAKKLAKLAK fused to the N-terminal protein transduction domain of translationally controlled tumor protein, MIIYRDLISH

Title
The cell penetrating ability of the proapoptotic peptide, KLAKLAKKLAKLAK fused to the N-terminal protein transduction domain of translationally controlled tumor protein, MIIYRDLISH
Authors
Kim H.Y.Kim S.Youn H.Chung J.-K.Shin D.H.Lee K.
Ewha Authors
이경림신동해
SCOPUS Author ID
이경림scopus; 신동해scopus
Issue Date
2011
Journal Title
Biomaterials
ISSN
0142-9612JCR Link
Citation
vol. 32, no. 22, pp. 5262 - 5268
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
We show here, that the proapoptotic peptide, KLAKLAKKLAKLAK (KLA), which by itself does not penetrate cell membranes, can do so when fused to a protein transduction domain derived from NH2-terminus of translationally controlled tumor protein (TCTP-PTD, MIIYRDLISH). Once inside the cell, the conjugated KLA exerts its proapoptotic activity to inhibit tumor growth. We evaluated the cellular uptake of KLA fused to TCTP-PTD (hereafter called TCTP-KLA) and its effect on cancer cell viability. The IC50 of TCTP-KLA was between 7 and 10 μmol/L. We also evaluated its anti-tumor activity in vivo by injecting it into xenografts of lung carcinoma in Balb/c nude mice. Tumor growth inhibition resulting from treatment with TCTP-KLA was better than that of TAT-KLA. These results suggest that TCTP-KLA can be applied to design cancer therapeutics. © 2011 Elsevier Ltd.
DOI
10.1016/j.biomaterials.2011.03.074
Appears in Collections:
약학대학 > 약학과 > Journal papers
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