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Isolation and characterization of microsatellite loci in the Korean crayfish, Cambaroides similis and application to natural population analysis

Title
Isolation and characterization of microsatellite loci in the Korean crayfish, Cambaroides similis and application to natural population analysis
Authors
Ahn D.H.Park M.H.Jung J.H.Oh M.J.Kim S.Jung J.Min G.S.
Ewha Authors
정종우
SCOPUS Author ID
정종우scopus
Issue Date
2011
Journal Title
Animal Cells and Systems
ISSN
1976-8354JCR Link
Citation
vol. 15, no. 1, pp. 37 - 43
Indexed
SCIE; SCOPUS; KCI WOS scopus
Abstract
The Korean freshwater crayfish, Cambaroides similis, has recently suffered from range reduction and habitat degradation caused by environmental changes and water pollution. For the conservation and restoration of this species, it is necessary to understand the current population structures of Korean C. similis using estimation of their genetic variation. In this study, eight microsatellite loci were developed and characterized from 49 individuals collected from four locations: one population from Mt. Bukhan (BH) and three populations from Mt. Gwanak (GA) in Seoul, Korea. As a result, the number of alleles per locus ranged from 2 to 12. The observed heterozygosities and expected heterozygosities ranged from 0.000 to 0.833 and from 0.125 to 0.943, respectively, and the former values were significantly lower than the latter ones expected under the Hardy-Weinberg equilibrium. No significant linkage disequilibrium was revealed between any of the locus pairs after Bonferroni correction. From the pairwise Fst results over all samples, higher differentiation between GA-BH population pairs (mean 0.1789) was observed than between GA population pairs (mean 0.0454). This was also supported by Mantel's test showing that the genetic distances of these crayfish populations were significantly correlated with geographic distances. This result may show the regional differentiation caused by restricted gene flow between northern (BH) and southern (GA) populations within Seoul. These microsatellite markers have the potential for use in analyses of the genetic diversity and population structure of C. similis species, with implications for its conservation and management plans. © 2011 Korean Society for Integrative Biology.
DOI
10.1080/19768354.2011.555137
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사범대학 > 과학교육과 > Journal papers
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