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Peroxiredoxin II restrains DNA damage-induced death in cancer cells by positively regulating JNK-dependent DNA repair

Title
Peroxiredoxin II restrains DNA damage-induced death in cancer cells by positively regulating JNK-dependent DNA repair
Authors
Lee K.W.Lee D.J.Lee J.Y.Kang D.H.Kwon J.Kang S.W.
Ewha Authors
강상원권종범이두재
SCOPUS Author ID
강상원scopus; 권종범scopus; 이두재scopus
Issue Date
2011
Journal Title
Journal of Biological Chemistry
ISSN
0021-9258JCR Link
Citation
vol. 286, no. 10, pp. 8394 - 8404
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
The 2-Cys peroxiredoxins (Prx) belong to a family of antioxidant enzymes that detoxify reactive oxygen and nitrogen species and are distributed throughout the intracellular and extracellular compartments. However, the presence and role of 2-Cys Prxs in the nucleus have not been studied. This study demonstrates that the PrxII located in the nucleus protects cancer cells from DNA damage-induced cell death. Although the two cytosolic 2-Cys Prxs, PrxI and PrxII, were found in the nucleus, only PrxII knockdown selectively and markedly increased cell death in the cancer cells treated with DNA-damaging agents. The increased death was completely reverted by the nuclearly targeted expression of PrxII in an activity-independent manner. Furthermore, the antioxidant butylated hydroxyanisole did not influence the etoposide-induced cell death. Mechanistically, the knockdown of Prx II expression impaired the DNA repair process by reducing the activation of the JNK/c-Jun pathway. These results suggest that PrxII is likely to be attributed to a tumor survival factor positively regulating JNK-dependent DNA repair with its inhibition possibly sensitizing cancer cells to chemotherapeutic agents. © 2011 by The American Society for Biochemistry and Molecular Biology, Inc.
DOI
10.1074/jbc.M110.179416
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자연과학대학 > 생명과학전공 > Journal papers
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