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Transforming growth factor-β1 bioassay involving matrix metalloproteinase-2 induction

Title
Transforming growth factor-β1 bioassay involving matrix metalloproteinase-2 induction
Authors
Kim G.-H.Kang S.-Y.Kwak H.-J.Baek K.-M.Hong S.-H.Lee H.-J.Sohn Y.-W.
Ewha Authors
이화정
SCOPUS Author ID
이화정scopus
Issue Date
2010
Journal Title
Journal of Interferon and Cytokine Research
ISSN
1079-9907JCR Link
Citation
vol. 30, no. 9, pp. 667 - 672
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
The transforming growth factor-β1 (TGF-β1) bioassay developed in this study monitors increased luciferase activity in MCF10A cells containing the matrix metalloproteinase-2 (MMP-2) promoter with a luciferase reporter and treated with increasing TGF-β1 concentrations. The response was linear in the concentration range from 75 to 2,500pg/mL. The abilities of 3 types of TGF-β in inducing MMP-2 were different. The luciferase activity induced by TGF-β1 was about 2 times more than that by TGF-β2 and TGF-β3. The MMP-2 promoter bioassay showed greater reproducibility (coefficient of variation [CV] 10%) than the previously developed anticell proliferation assay of TF-1 cell (CV 16%) and the MMP-2 zymogram assay (CV 40%). Mary Ann Liebert, Inc.
DOI
10.1089/jir.2009.0058
Appears in Collections:
약학대학 > 약학과 > Journal papers
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