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Suppression of adipocyte differentiation by 15-methoxypinusolidic acid through inhibition of PPARγ activity

Title
Suppression of adipocyte differentiation by 15-methoxypinusolidic acid through inhibition of PPARγ activity
Authors
Lee Y.S.Sung S.H.Hong J.-H.Hwang E.S.
Ewha Authors
황은숙
SCOPUS Author ID
황은숙scopus
Issue Date
2010
Journal Title
Archives of Pharmacal Research
ISSN
0253-6269JCR Link
Citation
vol. 33, no. 7, pp. 1035 - 1041
Indexed
SCIE; SCOPUS; KCI WOS scopus
Abstract
Pinusolide and its derivative, 15-methoxypinusolidic acid (15-MPA) are diterpenoid compounds isolated from Biota orientalis, which has been used as a Korean folk medicine for treating inflammatory disorders. Pinusolide and 15-MPA suppress nitric oxide generation by suppressing inducible nitric oxide synthase and exerted anti-inflammatory functions, whereas other functions and regulatory mechanisms are largely unknown. In this study, we investigated whether pinusolide and 15-MPA modulate adipocyte differentiation from pre-adipocytes 3T3-L1 cells. We found that 15-MPA, not pinusolide, suppressed adipocyte differentiation in a dose-dependent manner, as revealed by lipid droplet formation and expression of adipogenic genes such as adiponectin and aP2. 15-MPA did not affect mRNA and protein levels of PPARγ, a key adipogenic transcription factor, whereas transcriptional activity of PPARγ was significantly attenuated by 15-MPA. While aP2 promoter activity was increased by ectopic overexpression of PPARγ or by rosiglitazone-induced endogenous PPARγ activation, PPARγ-induced aP2 promoter activity was inhibited in the presence of 15-MPA. These results suggest that 15-MPA suppresses adipocyte differentiation through the inhibition of PPARγ-dependent adipogenic gene expression. © 2010 The Pharmaceutical Society of Korea and Springer Netherlands.
DOI
10.1007/s12272-010-0709-0
Appears in Collections:
약학대학 > 약학과 > Journal papers
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