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Production of xylitol from d-xylose and glucose with recombinant Corynebacterium glutamicum

Title
Production of xylitol from d-xylose and glucose with recombinant Corynebacterium glutamicum
Authors
Kim S.-H.Yun J.-Y.Kim S.-G.Seo J.-H.Park J.-B.
Ewha Authors
박진병
SCOPUS Author ID
박진병scopus
Issue Date
2010
Journal Title
Enzyme and Microbial Technology
ISSN
0141-0229JCR Link
Citation
vol. 46, no. 5, pp. 366 - 371
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
The product spectrum of a soil bacterium Corynebacterium glutamicum was extended to include a functional sugar xylitol. The recombinant C. glutamicum, engineered to express the xylose reductase gene XYL1of Pichia stipitis, produced xylose reductase with a specific activity of ca. 0.6. U/mg protein. Due to the absence of xylose isomerase and xylitol dehydrogenase genes, loose catabolite repression, high NADPH regeneration capacity, and tolerance against sugar-induced osmotic stress, the recombinant biocatalyst was able to efficiently produce xylitol from d-xylose using glucose as source of reducing equivalents. A fed-batch culture-based biotransformation allowed xylitol to accumulate to a concentration of 34.4. g/L (226. mM) in the medium with the specific productivity and product yield of xylose of 0.092. g/g dry cells/h and over 97%, respectively. The molar yield of xylitol to energy source during the biotransformation reached approximately 1.6. mol of xylose/mol of glucose. © 2009 Elsevier Inc.
DOI
10.1016/j.enzmictec.2009.12.012
Appears in Collections:
엘텍공과대학 > 식품공학전공 > Journal papers
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