Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박진병 | * |
dc.date.accessioned | 2016-08-28T12:08:57Z | - |
dc.date.available | 2016-08-28T12:08:57Z | - |
dc.date.issued | 2010 | * |
dc.identifier.issn | 0141-0229 | * |
dc.identifier.other | OAK-6368 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/220616 | - |
dc.description.abstract | The product spectrum of a soil bacterium Corynebacterium glutamicum was extended to include a functional sugar xylitol. The recombinant C. glutamicum, engineered to express the xylose reductase gene XYL1of Pichia stipitis, produced xylose reductase with a specific activity of ca. 0.6. U/mg protein. Due to the absence of xylose isomerase and xylitol dehydrogenase genes, loose catabolite repression, high NADPH regeneration capacity, and tolerance against sugar-induced osmotic stress, the recombinant biocatalyst was able to efficiently produce xylitol from d-xylose using glucose as source of reducing equivalents. A fed-batch culture-based biotransformation allowed xylitol to accumulate to a concentration of 34.4. g/L (226. mM) in the medium with the specific productivity and product yield of xylose of 0.092. g/g dry cells/h and over 97%, respectively. The molar yield of xylitol to energy source during the biotransformation reached approximately 1.6. mol of xylose/mol of glucose. © 2009 Elsevier Inc. | * |
dc.language | English | * |
dc.title | Production of xylitol from d-xylose and glucose with recombinant Corynebacterium glutamicum | * |
dc.type | Article | * |
dc.relation.issue | 5 | * |
dc.relation.volume | 46 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 366 | * |
dc.relation.lastpage | 371 | * |
dc.relation.journaltitle | Enzyme and Microbial Technology | * |
dc.identifier.doi | 10.1016/j.enzmictec.2009.12.012 | * |
dc.identifier.wosid | WOS:000275539400006 | * |
dc.identifier.scopusid | 2-s2.0-77649190270 | * |
dc.author.google | Kim S.-H. | * |
dc.author.google | Yun J.-Y. | * |
dc.author.google | Kim S.-G. | * |
dc.author.google | Seo J.-H. | * |
dc.author.google | Park J.-B. | * |
dc.contributor.scopusid | 박진병(15036390700) | * |
dc.date.modifydate | 20240322114808 | * |