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Suppression of inducible nitric oxide synthase by (-)-isoeleutherin from the bulbs of Eleutherine americana through the regulation of NF-κB activity

Title
Suppression of inducible nitric oxide synthase by (-)-isoeleutherin from the bulbs of Eleutherine americana through the regulation of NF-κB activity
Authors
Song S.-H.Min H.-Y.Han A.-R.Nam J.-W.Seo E.-K.Seoung Woo ParkSang Hyung LeeSang Kook Lee
Ewha Authors
이상국서은경한아름
SCOPUS Author ID
서은경scopus; 한아름scopusscopus
Issue Date
2009
Journal Title
International Immunopharmacology
ISSN
1567-5769JCR Link
Citation
vol. 9, no. 3, pp. 298 - 302
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Eleutherine americana Merr. et Heyne (Iridaceae) has been used as a folk medicine for the treatment of coronary disorders and wound-healing. In our previous phytochemical study, several pyranonaphthoquinoids, including (-)-isoeleutherin, were isolated from the bulbs of E. americana. Because inhibitors of inducible nitric oxide synthase (iNOS) might be useful as anti-inflammatory agents, we investigated the potential of pyranonaphthoquinoids to inhibit iNOS activity. We found that (-)-isoeleutherin inhibits lipopolysaccharide (LPS)-stimulated induction of nitric oxide (NO) in a dose-dependent manner (IC50 = 7.4 μM). Using western blots and reverse transcription-polymerase chain reaction, we showed that (-)-isoeleutherin suppresses the expression of iNOS protein and mRNA. In addition, (-)-isoeleutherin inhibited the expression of various cytokines such as interleukin-1β and interferon-β. Activation of the transcriptional activity of NF-κB by LPS was also inhibited by treatment with (-)-isoeleutherin, suggesting that (-)-isoeleutherin-mediated suppression of iNOS expression is associated with the regulation of transcription factor NF-κB. These findings suggest that (-)-isoeleutherin might be a novel anti-inflammatory agent, and that it may work by inhibiting NFkB activation in macrophages. © 2008 Elsevier B.V. All rights reserved.
DOI
10.1016/j.intimp.2008.12.003
Appears in Collections:
약학대학 > 약학과 > Journal papers
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