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Schisandra fructus extract ameliorates doxorubicin-induce cytotoxicity in cardiomyocytes: Altered gene expression for detoxification enzymes

Title
Schisandra fructus extract ameliorates doxorubicin-induce cytotoxicity in cardiomyocytes: Altered gene expression for detoxification enzymes
Authors
Choi E.H.Lee N.Kim H.J.Kim M.K.Chi S.-G.Kwon D.Y.Chun H.S.
Ewha Authors
김미경
SCOPUS Author ID
김미경scopusscopus
Issue Date
2008
Journal Title
Genes and Nutrition
ISSN
1555-8932JCR Link
Citation
vol. 2, no. 4, pp. 337 - 345
Indexed
SCIE; SCOPUS WOS scopus
Abstract
The effect of Schisandra fructus extract (SFE) on doxoruBicin (Dox)-induced cardiotoxicity was investigated in H9c2 cardiomyocytes. Dox, which is an antineoplastic drug known to induce cardiomyopathy possibly through production of reactive oxygen species, induced significant cytotoxicity, intracellular reactive oxygen species (ROS), and lipid peroxidation. SFE treatment significantly increased cell survival up to 25%, inhibited intracellular ROS production in a time- and dose-dependent manner, and inhibited lipid peroxidation induced by Dox. In addition, SFE treatment induced expression of cellular glutathione S-transferases (GSTs), which function in the detoxification of xenobiotics, and endogenous toxicants including lipid peoxides. Analyses of 31,100 genes using Affymetrix cDNA microarrays showed that SFE treatment up-regulated expression of genes involved in glutathione metabolism and detoxification [GST theta 1, mu 1, and alpha type 2, heme oxygenase 1 (HO-1), and microsomal epoxide hydrolase (mEH)] and energy metabolism [carnitine palmitoyltransferase-1 (CPT-1), transaldolase, and transketolase]. These data indicated that SFE might increase the resistance to cardiac cell injury by Dox, at least partly, together with altering gene expression, especially induction of phase II detoxification enzymes. © Springer-Verlag 2007.
DOI
10.1007/s12263-007-0073-y
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신산업융합대학 > 식품영양학과 > Journal papers
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