View : 19 Download: 0
Identification of a cytochrome P450 hydroxylase gene involved in rifamycin biosynthesis by Amycolatopsis mediterranei S699
- Identification of a cytochrome P450 hydroxylase gene involved in rifamycin biosynthesis by Amycolatopsis mediterranei S699
- Lee S.-K.; Choi C.-Y.; Ahn J.-S.; Cho J.-Y.; Park C.-S.; Yoon Y.J.
- Ewha Authors
- SCOPUS Author ID
- Issue Date
- Journal Title
- Journal of Microbiology and Biotechnology
- vol. 14, no. 2, pp. 356 - 362
- SCIE; SCOPUS; KCI
- In analyzing the region of the Amycolatopsis mediterranei S699 chromosome responsible for the biosynthesis of the ansamycin antibiotic rifamycin, we identified a gene, designated orf0, which is located immediately upstream of the rifamycin polyketide synthase (PKS). Orf0 encodes a protein, on the basis of sequence-comparative analysis, that is similar to several cytochrome P450 monooxygenases from different sources. The rifamycin producer, A. mediterranei, predominantly produces rifamycin B from its macrocyclic intermediate, proansamycin X, through dehydrogenation and hydroxylation steps. However, an A. mediterranei strain, deleted in orf0 by gene replacement, no longer produced rifamycin B. Furthermore, a versatile replicative vector in A. mediterranei was constructed and rifamycin B production was restored in a complementation experiment of orf0 using this novel vector. These consecutive results verified that the Orf0 protein, which is a P450 hydroxylase, is required for the production of rifamycin B in A. mediterranei. © The Korean Society for Microbiology and Biotechnology.
- Appears in Collections:
- 자연과학대학 > 화학·나노과학전공 > Journal papers
- Files in This Item:
There are no files associated with this item.
- RIS (EndNote)
- XLS (Excel)
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.