Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 윤여준 | - |
dc.date.accessioned | 2016-08-28T11:08:39Z | - |
dc.date.available | 2016-08-28T11:08:39Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.other | OAK-2144 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/219423 | - |
dc.description.abstract | In analyzing the region of the Amycolatopsis mediterranei S699 chromosome responsible for the biosynthesis of the ansamycin antibiotic rifamycin, we identified a gene, designated orf0, which is located immediately upstream of the rifamycin polyketide synthase (PKS). Orf0 encodes a protein, on the basis of sequence-comparative analysis, that is similar to several cytochrome P450 monooxygenases from different sources. The rifamycin producer, A. mediterranei, predominantly produces rifamycin B from its macrocyclic intermediate, proansamycin X, through dehydrogenation and hydroxylation steps. However, an A. mediterranei strain, deleted in orf0 by gene replacement, no longer produced rifamycin B. Furthermore, a versatile replicative vector in A. mediterranei was constructed and rifamycin B production was restored in a complementation experiment of orf0 using this novel vector. These consecutive results verified that the Orf0 protein, which is a P450 hydroxylase, is required for the production of rifamycin B in A. mediterranei. © The Korean Society for Microbiology and Biotechnology. | - |
dc.language | English | - |
dc.title | Identification of a cytochrome P450 hydroxylase gene involved in rifamycin biosynthesis by Amycolatopsis mediterranei S699 | - |
dc.type | Article | - |
dc.relation.issue | 2 | - |
dc.relation.volume | 14 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.index | KCI | - |
dc.relation.startpage | 356 | - |
dc.relation.lastpage | 362 | - |
dc.relation.journaltitle | Journal of Microbiology and Biotechnology | - |
dc.identifier.wosid | WOS:000221188800021 | - |
dc.identifier.scopusid | 2-s2.0-2442447199 | - |
dc.author.google | Lee S.-K. | - |
dc.author.google | Choi C.-Y. | - |
dc.author.google | Ahn J.-S. | - |
dc.author.google | Cho J.-Y. | - |
dc.author.google | Park C.-S. | - |
dc.author.google | Yoon Y.J. | - |
dc.contributor.scopusid | 윤여준(7402126465) | - |
dc.date.modifydate | 20210708161345 | - |